Characterization of six ampeloviruses infecting pineapple in Reunion Island using a combination of high-throughput sequencing approaches

Massé Delphine, Candresse Thierry, Filloux Denis, Massart Michel, Cassam Nathalie, Hostachy Bruno, Marais Armelle, Fernandez Emmanuel, Roumagnac Philippe, Verdin Eric, Teycheney Pierre-Yves, Lett Jean-Michel, Lefeuvre Pierre. 2024. Characterization of six ampeloviruses infecting pineapple in Reunion Island using a combination of high-throughput sequencing approaches. Viruses, 16 (7):1146, 17 p.

https://doi.org/10.3390/v16071146

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Résumé : The cultivation of pineapple (Ananas comosus) is threatened worldwide by mealybug wilt disease of pineapple (MWP), whose etiology is not yet fully elucidated. In this study, we characterized pineapple mealybug wilt-associated ampeloviruses (PMWaVs, family Closteroviridae) from a diseased pineapple plant collected from Reunion Island, using a high-throughput sequencing approach combining Illumina short reads and Nanopore long reads. Reads co-assembly resulted in complete or near-complete genomes for six distinct ampeloviruses, including the first complete genome of pineapple mealybug wilt-associated virus 5 (PMWaV5) and that of a new species tentatively named pineapple mealybug wilt-associated virus 7 (PMWaV7). Short reads data provided high genome coverage and sequencing depths for all six viral genomes, contrary to long reads data. The 5′ and 3′ ends of the genome for most of the six ampeloviruses could be recovered from long reads, providing an alternative to RACE-PCRs. Phylogenetic analyses did not unveil any geographic structuring of the diversity of PMWaV1, PMWaV2 and PMWaV3 isolates, supporting the current hypothesis that PMWaVs were mainly spread by human activity and vegetative propagation.

Mots-clés Agrovoc : Ananas comosus, virus des végétaux, génome, maladie des plantes, séquence nucléotidique, Closteroviridae, multiplication végétative, étiologie

Mots-clés géographiques Agrovoc : France, La Réunion

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission, European Regional Development Fund

Agences de financement hors UE : Conseil Régional de La Réunion, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail

Projets sur financement : (EU) INTERREG

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