The dark pigment in the sesame (Sesamum indicum L.) seed coat: Isolation, characterization, and its potential precursors

Dossou Senouwa Segla Koffi, Luo Zishu, Wang Zhijian, Zhou Wangyi, Zhou Rong, Zhang Yanxin, Li Donghua, Liu Aili, Dossa Komivi, You Jun, Wang Linhai. 2022. The dark pigment in the sesame (Sesamum indicum L.) seed coat: Isolation, characterization, and its potential precursors. Frontiers in Nutrition, 9:858673, 11 p.

https://doi.org/10.3389/fnut.2022.858673

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
611105.pdf
Télécharger (6MB) | Prévisualisation

Résumé : Sesame is a worldwide oilseed crop used in the food pharmacy. Its seed phenotypes determine the seed quality values. However, a thorough assessment of seed coat metabolites is lacking, and the dark pigment in the seed coat is not well-characterized. Herein, we report the isolation of melanin by the alkali method from the black and brown sesame seeds. Physicochemical methods, including scanning electron microscopy (SEM), solubility, precipitation, UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and thermogravimetric-differential scanning calorimetry (TG-DSC), were used to characterize the sesame melanins. The results clearly showed that the isolated pigments were similar to melanin from other sources. Both melanins were heat-stable and exhibited numerous characteristic absorption peaks. Through a comprehensible LC-MS/MS-based metabolome profiles analysis of NaOH and methanol extracts of black and white sesame seeds, caffeic, protocatechuic, indole-carboxylic, homogentisic, ferulic, vanillic, and benzoic acids were identified as the potential precursors of the sesame melanin. Our findings widen our understanding of dark seeds pigmentation in sesame. Furthermore, they show that black sesame seeds are promising sources of edible melanin for food and biotechnological applications.

Mots-clés Agrovoc : Sesamum indicum, caractère agronomique, mélanine, pelliculage des semences, acide benzoïque

Mots-clés libres : Sesame, Melanin, Seed coat color, Metabolite profiling, LC-MS/MS

Agences de financement hors UE : Chinese Academy of Agricultural Sciences

Auteurs et affiliations