Comparative study of two Rift Valley fever virus field strains originating from Mauritania

Chabert Mehdi, Lacote Sandra, Marianneau Philippe, Confort Marie-Pierre, Aurine Noémie, Pedarrieu Aurélie, Doumbia Baba, Gueya Mohamed Ould Baba Ould, Habiboullah Habiboullah, El Mamy Beyatt Ahmed Bezeid, Lo Modou Moustapha, Nichols Jenna, Sreenu Vattipally B., da Silva Filipe Ana, Colle Marie-Anne, Pain Bertrand, Cetre-Sossah Catherine, Arnaud Frédérick, Ratinier Maxime. 2024. Comparative study of two Rift Valley fever virus field strains originating from Mauritania. PLoS Neglected Tropical Diseases, 18 (12):e0012728, 34 p.

https://doi.org/10.1371/journal.pntd.0012728

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
2024_Chabert Ben Cherifa_Ratinier_Cetre Sossah_comparative study RVF mice.pdf
Télécharger (4MB) | Prévisualisation

Url - jeu de données - Entrepôt autre : https://figshare.com/articles/dataset/Numerical_data_used_to_generate_Figs_2_3_4_5_6_S5_and_S6_/27996049

Résumé : Rift Valley fever (RVF) is one of the major viral arthropod-borne diseases in Africa. In recent decades, RVF virus (RVFV), the causative agent of RVF, has been responsible for multiple outbreaks in West Africa with important consequences on human and animal health. In particular, an outbreak occurred in 2010 after heavy rains in the desertic region of Adrar, Mauritania. It was characterized by the appearance of severe clinical signs among dromedary camels. Another one occurred in 2013–2014 across Senegal and the southern part of Mauritania. In this study, we characterized two RVFV field strains isolated during these two outbreaks. The first strain, MRU25010-30, was isolated from a camel (2010) while the second, MRU2687-3, was isolated from a goat (2013). By deep-sequencing and rapid amplification of cDNA-ends by polymerase chain reaction, we successfully sequenced the complete genome of these two RVFV strains as well as the reference laboratory strain ZH548. Phylogenetic analysis showed that the two field viruses belong to two different RVFV genetic lineages. Moreover, we showed that MRU25010-30 replicates more efficiently in various in vitro cell culture models than MRU2687-3 and ZH548. In vivo, MRU25010-30 caused rapid death of BALB/c mice and proved to be more virulent than MRU2687-3, regardless of the route of inoculation (subcutaneous or intranasal). The virulence of MRU25010-30 is associated with a high viral load in the liver and serum of infected mice, while the death of mice infected with MRU2687-3 and ZH548 correlated with a high viral load in the brain. Altogether, the data presented in this study provide new avenues to unveil the molecular viral determinants that modulate RVFV virulence and replication capacity.

Mots-clés Agrovoc : Virus de la fièvre de la vallée du Rift, fièvre de la Vallée du Rift, transmission des maladies, dromadaire, variation génétique, génome, phylogénie, virus des animaux, PCR, épidémiologie, Enquête pathologique

Mots-clés géographiques Agrovoc : Mauritanie

Mots-clés libres : Virology, Mauritania, Rift Valley fever

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Institut Sénégalais de Recherches Agricole, Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Projets sur financement : (FRA) FORESEE

Auteurs et affiliations