Sequence typing of Bartonella henselae in small Indian mongooses (Urva auropunctata)

Muller Amanda, Maggi Ricardo, Sepulveda‑Garcia Paulina, Mau Alex, Sauvé Caroline, Conan Anne, Branford Ian, Bittencourt Pedro, Breitschwerdt Edward. 2024. Sequence typing of Bartonella henselae in small Indian mongooses (Urva auropunctata). Scientific Reports, 14:18654, 8 p.

Url - éditeur : https://www.nature.com/articles/s41598-024-69909-z

Résumé : This study aimed to determine the sequence type (ST) of Bartonella henselae infecting small Indian mongooses from Saint Kitts via multi‑locus sequence typing (MLST). This investigation used stored EDTA blood (n = 22) samples from mongooses previously identified as positive for B. henselae. Chocolate agar plates were enriched with Bartonella alpha‑Proteobacteria growth medium (BAPGM) to culture and isolate Bartonella from the blood samples. To perform MLST, DNA was extracted and purified from isolates followed by amplification by conventional PCR (300–500 bp) for eight genes (16S rDNA, batR, gltA, groEL, ftsZ, nlpD, ribC, and rpoB). Bartonella henselae STs were deposited in the PubMLST repository. Out of 22 B. henselae‑positive blood samples, isolates were obtained from 12 mongooses (54.5% ; 12/22). Each mongoose was infected with one ST. The studied mongoose population was infected with sequence types ST2, ST3, ST8, and a novel ST represented by ST38. Bartonella henselae ST2, ST3 and ST8 infecting mongooses are known to circulate in humans and cats, with ST2 and ST8 associated with Cat Scratch Disease (bartonellosis) in humans. The results presented herein denote the circulation of B. henselae STs with zoonotic potential in mongooses with risk of B. henselae transmission to humans.

Mots-clés Agrovoc : Bartonella henselae, mangouste, bartonella, séquence nucléotidique, phylogénie, PCR, transmission des maladies, identification, chat, sang, maladie de l'homme

Mots-clés géographiques Agrovoc : Caraïbes

Agences de financement hors UE : Ross University School of Veterinary Medicine

Auteurs et affiliations

• Muller Amanda, Ross University School of Veterinary Medicine (KNA) - auteur correspondant
• Maggi Ricardo, North Carolina State University (USA)
• Sepulveda‑Garcia Paulina, Universidad Austral de Chile (CHL)
• Mau Alex, UC Davis (USA)
• Sauvé Caroline, Université de Montréal (CAN)
• Conan Anne, CIRAD-BIOS-UMR ASTRE (ZWE) ORCID: 0000-0001-6108-3346
• Branford Ian, Ross University School of Veterinary Medicine (KNA)
• Bittencourt Pedro, Ross University School of Veterinary Medicine (KNA)
• Breitschwerdt Edward, North Carolina State University (USA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/612048/)

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