Co-acquired nanovirus and geminivirus exhibit a contrasted localization within their common aphid vector

Di Mattia Jeremy, Ryckebusch Faustine, Vernerey Marie-Stéphanie, Pirolles Elodie, Sauvion Nicolas, Peterschmitt Michel, Zeddam Jean-Louis, Blanc Stéphane. 2020. Co-acquired nanovirus and geminivirus exhibit a contrasted localization within their common aphid vector. Viruses, 12 (3):299, 14 p.

https://doi.org/10.3390/v12030299

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Quartile : Q2, Sujet : VIROLOGY

Résumé : Single-stranded DNA (ssDNA) plant viruses belong to the families Geminiviridae and Nanoviridae. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppers, whiteflies and aphids, nanoviruses are transmitted exclusively by aphids. Circulative transmission involves complex virus–vector interactions in which epithelial cells have to be crossed and defense mechanisms counteracted. Vector taxa are considered a relevant taxonomic criterion for virus classification, indicating that viruses can evolve specific interactions with their vectors. Thus, we predicted that, although nanoviruses and geminiviruses represent related viral families, they have evolved distinct interactions with their vector. This prediction is also supported by the non-structural Nuclear Shuttle Protein (NSP) that is involved in vector transmission in nanoviruses but has no similar function in geminiviruses. Thanks to the recent discovery of aphid-transmitted geminiviruses, this prediction could be tested for the geminivirus alfalfa leaf curl virus (ALCV) and the nanovirus faba bean necrotic stunt virus (FBNSV) in their common vector, Aphis craccivora. Estimations of viral load in midgut and head of aphids, precise localization of viral DNA in cells of insect vectors and host plants, and virus transmission tests revealed that the pathway of the two viruses across the body of their common vector differs both quantitatively and qualitatively.

Mots-clés Agrovoc : virus des végétaux, Geminiviridae, vecteur de maladie, Aphis craccivora, virus ADNsb, adn

Mots-clés libres : Fluorescent in situ hybridization, QPCR, Leaf discs, Hemiptera, Nanovirus, Geminivirus, Vector transmission

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Aspects moléculaires et cellulaires du cycle de vie des virus multipartites: les nanovirus

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