Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions

Durand Erwann, Lecomte Jérôme, Baréa Bruno, Piombo Georges, Dubreucq Eric, Villeneuve Pierre. 2012. Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions. Process Biochemistry (1991), 47 (12) : 2081-2089.

https://doi.org/10.1016/j.procbio.2012.07.027

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Quartile : Q1, Sujet : ENGINEERING, CHEMICAL / Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as 'green solvents' for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence of substrate polarity was assessed using alcohols of various chain lengths. Results showed that grinding of immobilized lipase was essential parameters for good lipase activity. Moreover, in our model reaction some hydrogen-bond donor component from the DES can compete with the alcoholysis reaction. Indeed, side reactions were observed with DES based on dicarboxylic acid or ethylene glycol, leading to some limitations of their use. However, the results showed that other DESs such as choline chloride:urea and choline chloride:glycerol could exhibit high activity and selectivity making them promising solvents for lipase-catalyzed reactions. Finally, the best DES's specific activity - and stability up to five days incubation time - were analyzed and compared with conventional organic solvents. Experiments revealed that iCALB is less influenced by the chain length of alcohol in DES than organic solvents and it is preserves its activity with minimally destructive to protein structure.

Mots-clés Agrovoc : Candida, biocatalyseur, estérification, solvant, propriété physicochimique, estérase, lipase

Mots-clés complémentaires : Candida antarctica, Transestérification

Classification Agris : 000 - Autres thèmes
Q02 - Traitement et conservation des produits alimentaires

Champ stratégique Cirad : Hors axes (2005-2013)

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