Occurrence of a novel mastrevirus in sugarcane germplasm collections in Florida, Guadeloupe and Réunion

Boukari Wardatou, Alcala-Briseño Ricardo I., Kraberger Simona, Fernandez Emmanuel, Filloux Denis, Daugrois Jean-Heinrich, Comstock Jack C., Lett Jean-Michel, Martin Darren Patrick, Varsani Arvind, Roumagnac Philippe, Polston Jane E., Rott Philippe. 2017. Occurrence of a novel mastrevirus in sugarcane germplasm collections in Florida, Guadeloupe and Réunion. Virology Journal, 14:146, 8 p.

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Quartile : Q3, Sujet : VIROLOGY

Abstract : Background: In Africa and Asia, sugarcane is the host of at least seven different virus species in the genus Mastrevirus of the family Geminiviridae. However, with the exception of Sugarcane white streak virus in Barbados, no other sugarcane-infecting mastrevirus has been reported in the New World. Conservation and exchange of sugarcane germplasm using stalk cuttings facilitates the spread of sugarcane-infecting viruses. Methods: A virion-associated nucleic acids (VANA)-based metagenomics approach was used to detect mastrevirus sequences in 717 sugarcane samples from Florida (USA), Guadeloupe (French West Indies), and Réunion (Mascarene Islands). Contig assembly was performed using CAP3 and sequence searches using BLASTn and BLASTx. Mastrevirus full genomes were enriched from total DNA by rolling circle amplification, cloned and sequenced. Nucleotide and amino acid sequence identities were determined using SDT v1.2. Phylogenetic analyses were conducted using MEGA6 and PHYML3. Results: We identified a new sugarcane-infecting mastrevirus in six plants sampled from germplasm collections in Florida and Guadeloupe. Full genome sequences were determined and analyzed for three virus isolates from Florida, and three from Guadeloupe. These six genomes share >88% genome-wide pairwise identity with one another and between 89 and 97% identity with a recently identified mastrevirus (KR150789) from a sugarcane plant sampled in China. Sequences similar to these were also identified in sugarcane plants in Réunion. Conclusions: As these virus isolates share <64% genome-wide identity with all other known mastreviruses, we propose classifying them within a new mastrevirus species named Sugarcane striate virus. This is the first report of sugarcane striate virus (SCStV) in the Western Hemisphere, a virus that most likely originated in Asia. The distribution, vector, and impact of SCStV on sugarcane production remains to be determined. (Résumé d'auteur)

Mots-clés Agrovoc : Saccharum officinarum, Virus des végétaux, Identification, génomique, Virologie, Taxonomie, Génome

Mots-clés géographiques Agrovoc : Guadeloupe, Floride, Réunion

Mots-clés complémentaires : Mastrevirus, Geminiviridae, Sugarcane striate virus, Émergence

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Agence(s) de financement européenne(s) : European Commission

Programme de financement européen : FP7

Projet(s) de financement européen(s) : A second metagenomics generation to better understand plant virus ecology and evolution

Auteurs et affiliations

  • Boukari Wardatou, University of Florida (USA)
  • Alcala-Briseño Ricardo I., University of Florida (USA)
  • Kraberger Simona, Arizona State University (USA)
  • Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Daugrois Jean-Heinrich, CIRAD-BIOS-UMR BGPI (FRA)
  • Comstock Jack C., USDA (USA)
  • Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU)
  • Martin Darren Patrick, UCT (ZAF)
  • Varsani Arvind, UCT (ZAF)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Polston Jane E., University of Florida (USA)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159

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