Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS)

Achir Nawel, Servent Adrien, Soto Marvin, Dhuique-Mayer Claudie. 2022. Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS). Journal of Spectroscopy:4509523, 13 p.

Résumé : A fast and low-cost analytical method to determine the concentrations of carotenoids (β-carotene, lutein, and lycopene) from mixed standard solutions or from fruit extracts (kiwi fruits, tomato paste, pink grapefruit juice, kiwi-pineapple smoothie, and apricot nectar) was tested. The methodology was based on UV-Vis spectrophotometry and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS). Results showed that relative concentrations of β-carotene, lutein, and lycopene in solvent were successfully determined by this technique with an error inferior to 6%. In real extracts, the procedure succeeded well in identifying the major carotenoid type of the fruit samples but also a more complex profile of a fruit mixture. The results also showed that accuracy of carotenoids determination by UV-Vis spectrophotometry-MCR-ALS in fruit extracts was conditioned by their spectral characteristics (III/II ratios and λmax), their relative proportion, and the extract purity.

Mots-clés Agrovoc : composition des aliments, analyse quantitative, caroténoïde, extrait de fruit, spectrophotométrie

Classification Agris : Q04 - Composition des produits alimentaires
Q01 - Sciences et technologies alimentaires - Considérations générales

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Auteurs et affiliations

• Achir Nawel, Montpellier SupAgro (FRA) - auteur correspondant
• Servent Adrien, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-9670-6707
• Soto Marvin, Université de Montpellier (FRA)
• Dhuique-Mayer Claudie, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0001-7153-2897

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600261/)

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