Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS)

Achir Nawel, Servent Adrien, Soto Marvin, Dhuique-Mayer Claudie. 2022. Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS). Journal of Spectroscopy:4509523, 13 p.

https://doi.org/10.1155/2022/4509523

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Résumé : A fast and low-cost analytical method to determine the concentrations of carotenoids (β-carotene, lutein, and lycopene) from mixed standard solutions or from fruit extracts (kiwi fruits, tomato paste, pink grapefruit juice, kiwi-pineapple smoothie, and apricot nectar) was tested. The methodology was based on UV-Vis spectrophotometry and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS). Results showed that relative concentrations of β-carotene, lutein, and lycopene in solvent were successfully determined by this technique with an error inferior to 6%. In real extracts, the procedure succeeded well in identifying the major carotenoid type of the fruit samples but also a more complex profile of a fruit mixture. The results also showed that accuracy of carotenoids determination by UV-Vis spectrophotometry-MCR-ALS in fruit extracts was conditioned by their spectral characteristics (III/II ratios and λmax), their relative proportion, and the extract purity.

Mots-clés Agrovoc : composition des aliments, analyse quantitative, caroténoïde, extrait de fruit, spectrophotométrie

Classification Agris : Q04 - Composition des produits alimentaires
Q01 - Sciences et technologies alimentaires - Considérations générales

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

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