Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals

Mwakasungula Solomon, Rougeron Virginie, Arnathau Céline, Boundenga Larson, Miguel Eve, Boissière Manuel, Jiolle Davy, Durand Patrick, Msigwa Alphonce, Mswata Sarah, Olotu Ally, Sterkers Yvon, Roche Benjamin, Killeen Gerard, Cerqueira Frédérique, Bitome-Essono Paul Yannick, Bretagnolle François, Masanja Honorati, Paupy Christophe, Sumaye Robert, Prugnolle Franck. 2022. Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals. Molecular Ecology Resources, 22 (8) : 2915-2927.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA830652 / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/ON652617

Résumé : Many emerging infectious diseases originate from wild animals, so there is a profound need for surveillance and monitoring of their pathogens. However, the practical difficulty of sample acquisition from wild animals tends to limit the feasibility and effectiveness of such surveys. Xenosurveillance, using blood-feeding invertebrates to obtain tissue samples from wild animals and then detect their pathogens, is a promising method to do so. Here, we describe the use of tsetse fly blood meals to determine (directly through molecular diagnostic and indirectly through serology), the diversity of circulating blood-borne pathogens (including bacteria, viruses and protozoa) in a natural mammalian community of Tanzania. Molecular analyses of captured tsetse flies (182 pools of flies totalizing 1728 flies) revealed that the blood meals obtained came from 18 different vertebrate species including 16 non-human mammals, representing approximately 25% of the large mammal species present in the study area. Molecular diagnostic demonstrated the presence of different protozoa parasites and bacteria of medical and/or veterinary interest. None of the six virus species searched for by molecular methods were detected but an ELISA test detected antibodies against African swine fever virus among warthogs, indicating that the virus had been circulating in the area. Sampling of blood-feeding insects represents an efficient and practical approach to tracking a diversity of pathogens from multiple mammalian species, directly through molecular diagnostic or indirectly through serology, which could readily expand and enhance our understanding of the ecology and evolution of infectious agents and their interactions with their hosts in wild animal communities.

Mots-clés Agrovoc : transmission des maladies, animal sauvage, relation hôte pathogène, agent pathogène, écologie animale, surveillance épidémiologique, viande de gibier, maladie infectieuse, maladie des animaux, zoonose, virus peste porcine africaine, identification, Glossina, sérologie

Mots-clés géographiques Agrovoc : République-Unie de Tanzanie

Mots-clés libres : Blood meals, Invertebrate-derived DNA (iDNA), Tsetse flies, Wildlife infectious diseases, Xenosurveillance

Agences de financement européennes : European Commission

Agences de financement hors UE : Agence Nationale de la Recherche

Programme de financement européen : H2020

Projets sur financement : (EU) A global alliance for Zika virus control and prevention, (FRA) Mediterranean Center for Environment and Biodiversity, (FRA) Alliance française contre les maladies parasitaires

Auteurs et affiliations

• Mwakasungula Solomon, Ifakara Health Institute (TZA) - auteur correspondant
• Rougeron Virginie, CNRS (FRA) - auteur correspondant
• Arnathau Céline, CNRS (FRA)
• Boundenga Larson, CIRMF (GAB)
• Miguel Eve, CNRS (FRA)
• Boissière Manuel, CIRAD-ES-UPR Forêts et sociétés (FRA) ORCID: 0000-0002-2552-7664
• Jiolle Davy, CIRMF (GAB)
• Durand Patrick, CNRS (FRA)
• Msigwa Alphonce, Tanzania National Parks (TZA)
• Mswata Sarah, Ifakara Health Institute (TZA)
• Olotu Ally, Ifakara Health Institute (TZA)
• Sterkers Yvon, Université de Montpellier (FRA)
• Roche Benjamin, CNRS (FRA)
• Killeen Gerard, Ifakara Health Institute (TZA)
• Cerqueira Frédérique, Université de Montpellier (FRA)
• Bitome-Essono Paul Yannick, IRET (GAB)
• Bretagnolle François, Université de Bourgogne (FRA)
• Masanja Honorati, Ifakara Health Institute (TZA)
• Paupy Christophe, IRD (CMR)
• Sumaye Robert, Ifakara Health Institute (TZA)
• Prugnolle Franck, IRD (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/605750/)

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