EU-OSTID, a collection of transposon insertional mutants for functional genomics in rice

Van Enckevort J.G., Droc Gaëtan, Piffanelli Pietro, Greco Rafaella, Gagneur Cyril, Weber Christelle, Gonzalez Victor M., Cabot Pere, Fornara Fabio, Berri Stefano, Miro Berta, Lan Ping, Rafel Marta, Capell Teresa, Puigdomenech Pere, Ouwerkerk Pieter B.F., Meijer Annemarie H., Pè Enrico, Colombo Lucia, Christou Paul, Guiderdoni Emmanuel, Pereira Andy. 2005. EU-OSTID, a collection of transposon insertional mutants for functional genomics in rice. Plant Molecular Biology, 59 (1) : 99-110.

Résumé : A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.

Mots-clés Agrovoc : Oryza sativa, transposition de gènes, adn, mutant, génie génétique, collection de matériel génétique, gène, expression des gènes, transposon

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Van Enckevort J.G., PRI (NLD)
• Droc Gaëtan, CIRAD-AMIS-BIOTROP (FRA) ORCID: 0000-0003-1849-1269
• Piffanelli Pietro, CIRAD-AMIS-BIOTROP (FRA)
• Greco Rafaella, PRI (NLD)
• Gagneur Cyril
• Weber Christelle
• Gonzalez Victor M., CSIC (ESP)
• Cabot Pere, CSIC (ESP)
• Fornara Fabio, University of Milan (ITA)
• Berri Stefano, University of Milan (ITA)
• Miro Berta, Fraunhofer Institute of Molecular Biology and Applied Ecology (DEU)
• Lan Ping, Fraunhofer Institute of Molecular Biology and Applied Ecology (DEU)
• Rafel Marta, Fraunhofer Institute of Molecular Biology and Applied Ecology (DEU)
• Capell Teresa, Fraunhofer Institute of Molecular Biology and Applied Ecology (DEU)
• Puigdomenech Pere, Institute of Biology Leiden (NLD)
• Ouwerkerk Pieter B.F., Institute of Biology Leiden (NLD)
• Meijer Annemarie H., Institute of Biology Leiden (NLD)
• Pè Enrico, University of Milan (ITA)
• Colombo Lucia, University of Milan (ITA)
• Christou Paul, Fraunhofer Institute of Molecular Biology and Applied Ecology (DEU)
• Guiderdoni Emmanuel, CIRAD-AMIS-BIOTROP (FRA)
• Pereira Andy, PRI (NLD)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/529107/)

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