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Anthocyanin and flavonoid production from Perilla frutescens : pilot plant scale processing including cross-flow microfiltration and reverse osmosis

Meng Linghua, Lozano Yves, Bombarda Isabelle, Gaydou Emile, Bin Li. 2006. Anthocyanin and flavonoid production from Perilla frutescens : pilot plant scale processing including cross-flow microfiltration and reverse osmosis. Journal of Agricultural and Food Chemistry, 54 : 4297-4303.

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Résumé : Extraction and concentration at a pilot plant scale of anthocyanins and flavonoids from Perilla frutescens var. frutescens harvested in the Guangzhou area of China were investigated. The study of extraction efficiency using mineral acids and organic acids showed that 0.01 mol/L nitric acid was the most suitable to extract flavonoids from this slightly red leaf cultivar. The red extract contained 12 mg/L (as cyanidin equivalent) anthocyanins and other flavones. The multistep process included cross-flow microfiltration (CFM) with a ceramic type membrane, reverse osmosis (RO), and rotating evaporation (RE). The filtration fluxes were high and constant for CFM (150 L/h/m2 at 0.6 b) and for RO (22 L/h/m2 at 40 b). The red extract was concentrated 9.4 times by RO and then 5.4 times by RE. It contained 422 mg/L anthocyanins, representing 77% of the total extracted anthocyanin. The proportion of flavonoids was found unchanged during processing. The concentrated extract showed a pH of 2.7, and its free acidity was found to be 46% of the acidity added for extraction, because of the buffering capacity of the extract. At the concentration level reached, a crystallized deposit occurred and was identified as tartrate.

Mots-clés Agrovoc : Perilla frutescens, anthocyane, flavonoïde, biosynthèse, polyphénol, microfiltration, extraction, osmose inverse

Mots-clés géographiques Agrovoc : Chine

Classification Agris : Q05 - Additifs alimentaires
Q02 - Traitement et conservation des produits alimentaires
Q60 - Traitement des produits agricoles non alimentaires

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

  • Meng Linghua, CIRAD-AMIS-UMR GPEB (FRA)
  • Lozano Yves, CIRAD-AMIS-UMR GPEB (FRA)
  • Bombarda Isabelle, CNRS (FRA)
  • Gaydou Emile, CNRS (FRA)
  • Bin Li, SCAU (CHN)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/537219/)

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