Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera)

N'Nan Oulo, Hocher Valérie, Verdeil Jean-Luc, Konan Konan Jean Louis, Ballo Koffi C., Mondeil Fanja, Malaurie Bernard. 2008. Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera). CryoLetters, 29 (4) : 339-350.

Résumé : This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.

Mots-clés Agrovoc : Cocos nucifera, conservation biologique, congélation, encapsulation, déshydratation, plumule, tissu végétal, saccharose, régénération in vitro, pousse, cryoconservation

Mots-clés géographiques Agrovoc : Côte d'Ivoire

Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• N'Nan Oulo, IRD (FRA)
• Hocher Valérie, IRD (FRA)
• Verdeil Jean-Luc, CIRAD-BIOS-UMR DAP (FRA)
• Konan Konan Jean Louis, CNRA (CIV)
• Ballo Koffi C., CNRA (CIV)
• Mondeil Fanja, Université de Cocody (CIV)
• Malaurie Bernard, CIRAD-CP-UMR BEPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/546471/)

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