Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus

Ollitrault Frédérique, Terol Javier F., Pina José Antonio, Navarro Luis, Talon Manuel, Ollitrault Patrick. 2010. Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus. American Journal of Botany, 97 (11), e124-e129e

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. Methods and Results: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. Conclusions: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.

Mots-clés Agrovoc : Citrus, chromosome, marqueur génétique, microsatellite, carte génétique, Rutaceae, variation génétique, phylogénie, évolution, génotype, polymorphisme génétique, hybridation intraspécifique, hybridation interspécifique, Citrus clementina

Mots-clés complémentaires : SSR, Séquencage, Chromosome artificiel

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Ollitrault Frédérique, IVIA (ESP)
• Terol Javier F., IVIA (ESP)
• Pina José Antonio, IVIA (ESP)
• Navarro Luis, IVIA (ESP)
• Talon Manuel, IVIA (ESP)
• Ollitrault Patrick, CIRAD-BIOS-UPR Multiplication végétative (ESP) ORCID: 0000-0002-9456-5517

Source : Cirad - Agritrop (https://agritrop.cirad.fr/558018/)

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