The sugarcane genome challenge: Strategies for sequencing a highly complex genome

Mendes Souza Glaucia, Berges Hélène, Bocs Stéphanie, Casu Rosanne, D'Hont Angélique, Ferreira João Eduardo, Henry Robert, Ming Ray, Potier Bernard, Van Sluys Marie-Anne, Vincentz Michel, Paterson Andrew H.. 2011. The sugarcane genome challenge: Strategies for sequencing a highly complex genome. Tropical Plant Biology, 3 (3-4) : 145-156.

https://doi.org/10.1007/s12042-011-9079-0

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Résumé : Sugarcane cultivars derive from interspecific hybrids obtained by crossing Saccharum officinarum and Saccharum spontaneum and provide feedstock used worldwide for sugar and biofuel production. The importance of sugarcane as a bioenergy feedstock has increased interest in the generation of new cultivars optimised for energy production. Cultivar improvement has relied largely on traditional breeding methods, which may be limited by the complexity of inheritance in interspecific polyploid hybrids, and the time-consuming process of selection of plants with desired agronomic traits. In this sense, molecular genetics can assist in the process of developing improved cultivars by generating molecular markers that can be used in the breeding process or by introducing new genes into the sugarcane genome. For meeting each of these, and additional goals, biotechnologists would benefit from a reference genome sequence of a sugarcane cultivar. The sugarcane genome poses challenges that have not been addressed in any prior sequencing project, due to its highly polyploid and aneuploid genome structure with a complete set of homeologous genes predicted to range from 10 to 12 copies (alleles) and to include representatives from each of two different species. Although sugarcane's monoploid genome is about 1 Gb, its highly polymorphic nature represents another significant challenge for obtaining a genuine assembled monoploid genome. With a rich resource of expressed-sequence tag (EST) data in the public domain, the present article describes tools and strategies that may aid in the generation of a reference genome sequence.

Mots-clés Agrovoc : Saccharum, hybride, Saccharum spontaneum, Saccharum officinarum, Erianthus arundinaceus, Sorghum, bioinformatique, génome, amélioration des plantes, biocarburant

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Séquencage, Saccharum arundinaceum

Classification Agris : F30 - Génétique et amélioration des plantes
P06 - Sources d'énergie renouvelable

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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