Microsatellite markers for population studies of Phytophthora megakarya (Pythiaceae), a cacao pathogen in Africa

Mfegue Crescence Virginie, Herail Claude, Adreit Henri, Mbenoum Michael, Techou Zaccharie, Ten Hoopen Gerben Martijn, Tharreau Didier, Ducamp Michel. 2012. Microsatellite markers for population studies of Phytophthora megakarya (Pythiaceae), a cacao pathogen in Africa. American Journal of Botany, 99 (9), e353-e356

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Premise of the study: Phytophthora megakarya is the agent of black pod disease of cacao and is the main pathogen of this crop in Africa. Population genetic studies are required to investigate how this pathogen emerged. To this end, we developed 12 novel polymorphic microsatellite markers for P. megakarya. o Methods and Results: Microsatellite sequences were obtained by pyrosequencing of multiplex-enriched libraries. Candidate loci with di- or trinucleotide motifs were selected, and primer pairs were tested with nine P. megakarya isolates. The 12 most polymorphic and unambiguous loci were selected to develop three multiplex PCR pools. The total number of alleles varied from two to nine, depending on loci, and higher than expected heterozygosity was observed. o Conclusions: These markers were used for population genetic studies of P. megakarya in Cameroon and for comparison with reference strains from West Africa. This is the first time that microsatellite markers have been developed for P. megakarya.

Mots-clés Agrovoc : Phytophthora, Phytophthora palmivora, variation génétique, génétique des populations, microsatellite, phylogénie, Theobroma cacao

Mots-clés géographiques Agrovoc : Cameroun, Afrique centrale, Afrique occidentale

Mots-clés complémentaires : Phytophtora megakarya, Séquencage

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Mfegue Crescence Virginie, IRAD (CMR)
• Herail Claude, CIRAD-BIOS-UMR BGPI (FRA)
• Adreit Henri, CIRAD-BIOS-UMR BGPI (FRA)
• Mbenoum Michael, IRAD (CMR)
• Techou Zaccharie, IRAD (CMR)
• Ten Hoopen Gerben Martijn, CIRAD-BIOS-UPR Bioagresseurs (CMR) ORCID: 0000-0003-2133-3130
• Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
• Ducamp Michel, CIRAD-BIOS-UMR BGPI (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/565818/)

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