Cytological and molecular characterization of three gametoclones of Citrus clementina

Germanà Maria Antonietta, Aleza Pablo, Carrera Esther, Chen Chunxian, Chiancone Benedetta, Costantino Gilles, Dambier Dominique, Deng Xiuxin, Federici Claire T., Froelicher Yann, Guo Wenwu, Ibáñez Victoria, Juarez José, Kwok Kevin, Luro François, Machado Marcos Antonio, Naranjo Miguel Angel, Navarro Luis, Ollitrault Patrick, Rios Gabino, Roose Mikeal L., Talon Manuel, Xu Qiang, Gmitter Frederick G. Jr.. 2013. Cytological and molecular characterization of three gametoclones of Citrus clementina. BMC Plant Biology, 13 (1), 8 p.

https://doi.org/10.1186/1471-2229-13-129

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Url - jeu de données - Entrepôt autre : http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-3926/ / Url - jeu de données - Entrepôt autre : http://www.biomedcentral.com/1471-2229/13/129/additional

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (array-CGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a single peak (allele), were observed among the three plants at almost all SSR loci distributed across the entire diploid donor genome. Those few loci with extra peaks visualized as output from automated sequencing runs, generally low or ambiguous, might result from amplicons of paralogous members at the locus, non-specific sites, or unexpected recombinant alleles. No new alleles were found, suggesting the genomes remained stable and intact during gametogenesis and regeneration. The integrity of the haploid genome also was supported by array-CGH studies, in which genomic profiles were comparable to the diploid control. Conclusions The presence of few gene hybridization abnormalities, corroborated by gene dosage measurements, were hypothetically due to the segregation of hemizygous alleles and minor genomic rearrangements occurring during the haploidization procedure. In conclusion, these plants that are valuable genetic and breeding materials contain completely homozygous and essentially intact genomes.

Mots-clés Agrovoc : Citrus, culture d'anthère, gynogénèse, évolution, haploïdie, séquence nucléotidique, locus des caractères quantitatifs, gamète, biologie moléculaire, cytologie, Citrus clementina