The mitochondrial-targeted antioxidant, MitoQ, increases liver mitochondrial cardiolipin content in obesogenic diet-fed rats

Fouret Gilles, Tolika Evanthia, Lecomte Jérôme, Bonafos Béatrice, Aoun Manar, Murphy Michael P., Ferreri Carla, Chatgilialoglu Chryssostomos, Dubreucq Eric, Coudray Charles, Feillet-Coudray Christine. 2015. The mitochondrial-targeted antioxidant, MitoQ, increases liver mitochondrial cardiolipin content in obesogenic diet-fed rats. Biochimica and Biophysica Acta. Bioenergetics, 1847 (10) : 1025-1035.

Quartile : Q1, Sujet : BIOPHYSICS / Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : Cardiolipin (CL), a unique mitochondrial phospholipid, plays a key role in several processes of mitochondrial bioenergetics as well as in mitochondrial membrane stability and dynamics. The present study was designed to determine the effect of MitoQ, a mitochondrial-targeted antioxidant, on the content of liver mitochondrial membrane phospholipids, in particular CL, and its fatty acid composition in obesogenic diet-fed rats. To do this, twenty-four 6 week old male Sprague Dawley rats were randomized into three groups of 8 animals and fed for 8 weeks with either a control diet, a high fat diet (HF), or a HF diet with MitoQ (HF + MitoQ). Phospholipid classes and fatty acid composition were assayed by chromatographic methods in liver and liver mitochondria. Mitochondrial bioenergetic function was also evaluated. While MitoQ had no or slight effects on total liver fatty acid composition and phospholipid classes and their fatty acid composition, it had major effects on liver mitochondrial phospholipids and mitochondrial function. Indeed, MitoQ both increased CL synthase gene expression and CL content of liver mitochondria and increased 18:2n-6 (linoleic acid) content of mitochondrial phospholipids by comparison to the HF diet. Moreover, mitochondrial CL content was positively correlated to mitochondrial membrane fluidity, membrane potential and respiration, as well as to ATP synthase activity, while it was negatively correlated to mitochondrial ROS production. These findings suggest that MitoQ may decrease pathogenic alterations to CL content and profiles, thereby preserving mitochondrial function and attenuating the development of some of the features of metabolic syndrome in obesogenic diet-fed rats.

Mots-clés Agrovoc : antioxydant, rat, acide gras, mitochondrie, phosphatide, bioénergétique, cardiolipine, expression des gènes, composition chimique, régime alimentaire, foie

Classification Agris : L50 - Physiologie et biochimie animales

Champ stratégique Cirad : Axe 3 (2014-2018) - Alimentation durable

Auteurs et affiliations

• Fouret Gilles, INRA (FRA)
• Tolika Evanthia, ISOF (ITA)
• Lecomte Jérôme, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0001-9942-5858
• Bonafos Béatrice, INRA (FRA)
• Aoun Manar, ISOF (ITA)
• Murphy Michael P., MRC (GBR)
• Ferreri Carla, ISOF (ITA)
• Chatgilialoglu Chryssostomos, NCSR (GRC)
• Dubreucq Eric, Montpellier SupAgro (FRA)
• Coudray Charles, INRA (FRA)
• Feillet-Coudray Christine, INRA (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/576401/)

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