Innovative combination of spectroscopic techniques to reveal nanoparticle fate in a crop plant

Larue Camille, Castillo-Michel Hiram, Stein Ricardo J., Fayard Barbara, Pouyet Emeline, Villanova Julie, Magnin Valérie, Pradas del Real Ana-Elena, Trcera Nicolas, Legros Samuel, Sorieul Stephanie, Sarret Géraldine. 2016. Innovative combination of spectroscopic techniques to reveal nanoparticle fate in a crop plant. Spectrochimica Acta. Part B, Atomic Spectroscopy, 119 : 17-24.

Quartile : Q1, Sujet : SPECTROSCOPY

Résumé : Nanotechnology is the new industrial revolution of our century. Its development leads to an increasing use of nanoparticles and thus to their dissemination. Their fate in the environment is of great concern and especially their possible transfer in trophic chains might be an issue for food safety. However, so far our knowledge on this topic has been restricted by the lack of appropriate techniques to characterize their behavior in complex matrices. Here, we present in detail the use of cutting-edge beam-based techniques for nanoparticle in situ localization, quantification and speciation in a crop plant species (Lactuca sativa). Lettuce seedlings have been exposed to TiO2 and Ag nanoparticles and analyzed by inductively coupled plasma spectrometry, micro-particle induced X-ray emission coupled to Rutherford backscattering spectroscopy on nuclear microprobe, micro-X-ray fluorescence spectroscopy and X-ray absorption near edge structure spectroscopy. The benefits and drawbacks of each technique are discussed, and the types of information that can be drawn, for example on the translocation to edible parts, change of speciation within the plant, detoxification mechanisms, or impact on the plant ionome, are highlighted. Such type of coupled approach would be an asset for nanoparticle risk assessment.

Mots-clés Agrovoc : spectroscopie, nanotechnologie, technique analytique, identification, corps étrangers, spectroscopie aux rayons x, spectroscopie émission fluorescence, spectrométrie d'absorption atomique, plante de culture, Lactuca sativa, méthode statistique, analyse multivariée, biosécurité, dioxyde de titane, argent, pollution, polluant industriel

Mots-clés complémentaires : Nanoparticule, Sécurité des aliments

Mots-clés libres : Nanoparticle, Micro-X-ray fluorescence spectroscopy, X-ray absorption near edge spectroscopy, Micro-particle induced X-ray emission, Rutherford backscattering spectroscopy, Multivariate statistical analysis

Classification Agris : U30 - Méthodes de recherche
Q03 - Contamination et toxicologie alimentaires
P02 - Pollution

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Larue Camille, CNRS (FRA)
• Castillo-Michel Hiram, ESRF (FRA)
• Stein Ricardo J., Ruhr-Universität Bochum (DEU)
• Fayard Barbara, CNRS (FRA)
• Pouyet Emeline, ESRF (FRA)
• Villanova Julie, ESRF (FRA)
• Magnin Valérie, CNRS (FRA)
• Pradas del Real Ana-Elena, CNRS (FRA)
• Trcera Nicolas, SOLEIL synchrotron (FRA)
• Legros Samuel, CIRAD-PERSYST-UPR Recyclage et risque (SEN) ORCID: 0000-0001-7081-6679
• Sorieul Stephanie, Université de Bordeaux I (FRA)
• Sarret Géraldine, CNRS (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580567/)

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