Members of BTB Gene Family of Scaffold Proteins Suppress Nitrate Uptake and Nitrogen Use Efficiency

Araus Viviana, Vidal Elena A., Puelma Tomas, Alamos Simón, Mieulet Delphine, Guiderdoni Emmanuel, Gutiérrez Rodrigo A.. 2016. Members of BTB Gene Family of Scaffold Proteins Suppress Nitrate Uptake and Nitrogen Use Efficiency. Plant Physiology, 171 (2) : 1523-1532.

Quartile : Outlier, Sujet : PLANT SCIENCES

Résumé : Development of crops with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. However, achieving this goal has proven difficult since NUE is a complex trait encompassing physiological and developmental processes. We thought to tackle this problem by taking a systems biology approach to identify candidate target genes. First, we used a supervised machine-learning algorithm to predict a NUE gene network in Arabidopsis (Arabidopsis thaliana). Second, we identified BT2, a member of the Bric-a-Brac/Tramtrack/Broad gene family, as the most central and connected gene in the NUE network. Third, we experimentally tested BT2 for a role in NUE. We found NUE decreased in plants overexpressing BT2 gene compared to wild-type plants under limiting nitrate conditions. In addition, NUE increased compared to wild-type plants under low nitrate conditions in double mutant plants in bt2 and its closely related homolog bt1, indicating a functional redundancy of BT1 and BT2 for NUE. Expression of the nitrate transporter genes NRT2.1 and NRT2.4 increased in the bt1/bt2 double mutant compared to wild-type plants, with a concomitant 65% increase in nitrate uptake under low nitrate conditions. Similar to Arabidopsis, we found that mutation of the BT1/BT2 ortholog gene in rice (Oryza sativa) OsBT increased NUE by 20% compared to wild-type rice plants under low nitrogen conditions. These results indicate BT gene family members act as conserved negative regulators of nitrate uptake genes and NUE in plants and highlight them as prime targets for future strategies to improve NUE in crops.

Mots-clés Agrovoc : Arabidopsis thaliana, Oryza sativa, gène, identification, efficacité d'utilisation, azote, expression des gènes, automatisation, apprentissage, bioinformatique, méthodologie, absorption, nitrate

Classification Agris : F30 - Génétique et amélioration des plantes
U10 - Informatique, mathématiques et statistiques
U30 - Méthodes de recherche
F61 - Physiologie végétale - Nutrition

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Araus Viviana, Pontificia Universidad Catolica de Chile (CHL)
• Vidal Elena A., Pontificia Universidad Catolica de Chile (CHL)
• Puelma Tomas, Pontificia Universidad Catolica de Chile (CHL)
• Alamos Simón, Pontificia Universidad Catolica de Chile (CHL)
• Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• Gutiérrez Rodrigo A., Pontificia Universidad Catolica de Chile (CHL)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/581038/)

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