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DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India

Harrup Lara E., Laban Swathi, Purse Bethan V., Krishnamohan Reddy Yarabolu, Narasimha Reddy Yella, Munivenkatappa Byregowda Sonnahallipura, Kumar Naveen, Muniramaiah Purushotham Kondappa, Kowalli Shrikant, Prasad Minakshi, Prasad Gaya, Bettis Alison A., de Keyser Rien, Garros Claire, Gopurenko David, Bellis Glenn A., Labuschagne Karien, Mathieu Bruno, Carpenter Simon. 2016. DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India. Parasites and Vectors, 9 (461), 20 p.

Journal article ; Article de synthèse ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : PARASITOLOGY

Abstract : Background: Culicoides spp. biting midges transmit bluetongue virus (BTV), the aetiological agent of bluetongue (BT), an economically important disease of ruminants. In southern India, hyperendemic outbreaks of BT exert high cost to subsistence farmers in the region, impacting on sheep production. Effective Culicoides spp. monitoring methods coupled with accurate species identification can accelerate responses for minimising BT outbreaks. Here, we assessed the utility of sampling methods and DNA barcoding for detection and identification of Culicoides spp. in southern India, in order to provide an informed basis for future monitoring o:f their populations in the region. Methods Culicoides spp. collected from Tamil Nadu and Karnataka were used to construct a framework for future morphological identification in surveillance, based on sequence comparison of the DNA barcode region of the mitochondrial cytochrome c oxidase I (COI) gene and achieving quality standards defined by the Barcode of Life initiative. Pairwise catches of Culicoides spp. were compared in diversity and abundance between green (570 nm) and ultraviolet (UV) (390 nm) light emitting diode (LED) suction traps at a single site in Chennai, Tamil Nadu over 20 nights of sampling in November 2013. Results: DNA barcode sequences of Culicoides spp. were mostly congruent both with existing DNA barcode data from other countries and with morphological identification of major vector species. However, sequence differences symptomatic of cryptic species diversity were present in some groups which require further investigation. While the diversity of species collected by the UV LED Center for Disease Control (CDC) trap did not significantly vary from that collected by the green LED CDC trap, the UV CDC significantly outperformed the green LED CDC trap with regard to the number of Culicoides individuals collected. Conclusions: Morphological identification of the majority of potential vector species of Culicoides spp. samples within southern India appears relatively robust; however, potential cryptic species diversity was present in some groups requiring further investigation. The UV LED CDC trap is recommended for surveillance of Culicoides in southern India. (Résumé d'auteur)

Mots-clés Agrovoc : Culicoides, Vecteur de maladie, Génétique des populations, Surveillance épidémiologique, Fièvre catarrhale du mouton, arboviruses [EN], Identification, Technique analytique, ADN, PCR, Phylogénie, Empreinte ADN

Mots-clés géographiques Agrovoc : Inde

Mots-clés complémentaires : Code-barres génétique

Classification Agris : L73 - Animal diseases
L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Harrup Lara E., The Pirbright Institute (GBR)
  • Laban Swathi, Tamil Nadu Veterinary and animal Sciences University (IND)
  • Purse Bethan V., NERC (GBR)
  • Krishnamohan Reddy Yarabolu, Tamil Nadu Veterinary and animal Sciences University (IND)
  • Narasimha Reddy Yella, College of Veterinary and Animal Science (IND)
  • Munivenkatappa Byregowda Sonnahallipura, Animal Health and Veterinary (IND)
  • Kumar Naveen, Animal Health and Veterinary (IND)
  • Muniramaiah Purushotham Kondappa, Animal Health and Veterinary (IND)
  • Kowalli Shrikant, Animal Health and Veterinary (IND)
  • Prasad Minakshi, Lala Lajpat Rai University of Veterinary and Animal Sciences (IND)
  • Prasad Gaya, Lala Lajpat Rai University of Veterinary and Animal Sciences (IND)
  • Bettis Alison A., LSHTM (GBR)
  • de Keyser Rien, LSHTM (GBR)
  • Garros Claire, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0003-4378-5031
  • Gopurenko David, NSW Agriculture (AUS)
  • Bellis Glenn A., Department of Agriculture (AUS)
  • Labuschagne Karien, OVI (ZAF)
  • Mathieu Bruno, EID (FRA)
  • Carpenter Simon, The Pirbright Institute (GBR)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/581436/)

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