Durand Erwann, Delavault André, Bourlieu Claire, Lecomte Jérôme, Baréa Bruno, Figueroa-Espinoza Maria-Cruz, Decker Eric Andrew, Salaun Françoise Michel, Kergourlay Gilles, Villeneuve Pierre. 2017. Eleostearic phospholipids as probes to evaluate antioxidants efficiency against liposomes oxidation. Chemistry and Physics of Lipids, 209 : 19-28.
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Version publiée
- Anglais
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Quartile : Q2, Sujet : BIOPHYSICS / Quartile : Q3, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY
Résumé : Regardless of the applications: therapeutic vehicle or membrane model to mimic complex biological systems; it is of a great importance to develop simplified, reproducible and rapid model assays allowing for a relevant assessment of the liposomal membrane oxidation and therefore antioxidant activity of selected molecules. Here, we describe a new and high-throughput assay that we called “Vesicle Conjugated Autoxidizable Triene (VesiCAT)”. It is based on specific UV absorbance spectral properties of a new phospholipid probe, synthesized with natural conjugated eleostearic acid extracted from Tung oil. The VesiCAT assay has been developed with two different radical generators (2,2′-azobis(2-amidinopropane) dihydrochloride; AAPH and 2,2′-azobis(2,4-dimethylvaleronitrile); AMVN), producing a constant flux of oxidant species, either in membrane or in aqueous phase. This method appears very efficient in assessing the effect of various pure antioxidant molecules in their ability to preserve liposomes from oxidative degradation. In addition, the AAPH- and AMVN-induced oxidations offer the possibility of extracting different but complementary information with respect to the antioxidants efficacy.
Mots-clés Agrovoc : antioxydant, oxydation, développement biologique, huile d'abrasin, phosphatide, organe de véhicule, efficacité
Classification Agris : Q02 - Traitement et conservation des produits alimentaires
000 - Autres thèmes
Champ stratégique Cirad : Axe 3 (2014-2018) - Alimentation durable
Auteurs et affiliations
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Durand Erwann, CIRAD-PERSYST-UMR IATE (FRA)
ORCID: 0000-0002-0306-8081
- Delavault André
- Bourlieu Claire, INRA (FRA)
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Lecomte Jérôme, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0001-9942-5858
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Baréa Bruno, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0009-0007-5164-1632
- Figueroa-Espinoza Maria-Cruz, INRA (FRA)
- Decker Eric Andrew, University of Massachusetts (USA)
- Salaun Françoise Michel, Diana Pet Food (FRA)
- Kergourlay Gilles, Diana Pet Food (FRA)
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Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0003-1685-1494
Source : Cirad-Agritrop (https://agritrop.cirad.fr/585867/)
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