Enzymology, histological and ultrastructural effects of ar-turmerone on Culex pipiens pallens larvae

Liu Jia, Fernandez Diana, Gao Yanjin, Silvie Pierre, Gao Yongdong, Dai Guanghui. 2020. Enzymology, histological and ultrastructural effects of ar-turmerone on Culex pipiens pallens larvae. Insects, 11:336, 13 p.

https://doi.org/10.3390/insects11060336

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

Prévisualisation

Version publiée - Anglais
Sous licence

.
insects-11-00336 (1).pdf
Télécharger (3MB) | Prévisualisation

Quartile : Q1, Sujet : ENTOMOLOGY

Résumé : Our previous article demonstrated that ar-turmerone ((6S)-2-methyl-6-(4-methylphenyl)-2-hepten-4-one) extracted from Curcuma longa L. has a significant larvicidal activity against the fourth instar larvae of Culex pipiens pallens. To reveal the effects of ar-turmerone on C. pipiens pallens larvae, light microscopy and transmission electron microscopy were used to observe the histological and ultrastructure changes in muscle and digestive tissues of fourth instar larvae. It was also revealed by detecting the activity of the acetylcholinesterase (AChE) enzyme and three detoxifying enzymes, including carboxylesterase (CarE), glutathione-S-transferase (GST) and Cytochrome P450 monooxidases (P450). The observation under the light microscope showed that the larvae displayed a disruption of myofibril in ventral muscle cells, the disappearance of nucleolus in the malpighian tubule cells, and the exfoliation of the brush border in midgut epithelial cells, 24 h after treatment. The observation under the transmission electron microscope displayed disorganized Z-lines in the ventral muscle cells, and dissolved membrane of mitochondria, nuclear and endoplasmic reticulum in abdominal cells. The enzymatic activity results showed that ar-turmerone significantly increased the level of detoxifying enzymes, while the activity of AChE was not obviously affected. All the results suggest that the larvicidal mechanism of ar-turmerone is estimated to be stomach poison and the active sites might be the muscle and digestive tissues, and the mode of action of ar-turmerone may be unrelated to AChE.

Mots-clés Agrovoc : Culex pipiens, Curcuma longa, ultrastructure, phytochimie, extrait d'origine végétale, histologie végétale, enzymologie

Mots-clés libres : Plant extracts, Botanicals, Plant-based product, Mosquito, Curcuma, China

Classification Agris : F60 - Physiologie et biochimie végétale
L50 - Physiologie et biochimie animales

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations