The Wolbachia mobilome in Culex pipiens includes a putative plasmid

Reveillaud Julie, Bordenstein Sarah R., Cruaud Corinne, Shaiber Alon, Esen Özcan C., Weill Mylène, Makoundou Patrick, Lolans Karen, Watson Andrea R., Rakotoarivony Ignace, Bordenstein Seth R., Eren A. Murat. 2019. The Wolbachia mobilome in Culex pipiens includes a putative plasmid. Nature Communications, 10 (1):1051, 11 p.

Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Note générale : Corrigendum paru dans Nature Communications (2019)vol.10, article number 3153, 1 p., https://doi.org/10.1038/s41467-019-11234-5

Résumé : Wolbachia is a genus of obligate intracellular bacteria found in nematodes and arthropods worldwide, including insect vectors that transmit dengue, West Nile, and Zika viruses. Wolbachia's unique ability to alter host reproductive behavior through its temperate bacteriophage WO has enabled the development of new vector control strategies. However, our understanding of Wolbachia's mobilome beyond its bacteriophages is incomplete. Here, we reconstruct near-complete Wolbachia genomes from individual ovary metagenomes of four wild Culex pipiens mosquitoes captured in France. In addition to viral genes missing from the Wolbachia reference genome, we identify a putative plasmid (pWCP), consisting of a 9.23-kbp circular element with 14 genes. We validate its presence in additional Culex pipiens mosquitoes using PCR, long-read sequencing, and screening of existing metagenomes. The discovery of this previously unrecognized extrachromosomal element opens additional possibilities for genetic manipulation of Wolbachia.

Mots-clés Agrovoc : Wolbachia, génome, vecteur de maladie, Culex pipiens, plasmide

Mots-clés géographiques Agrovoc : France

Mots-clés complémentaires : Métagénomique

Mots-clés libres : Metagenomics, Mobile elements

Classification Agris : L72 - Organismes nuisibles des animaux
L10 - Génétique et amélioration des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Reveillaud Julie, INRA (FRA) - auteur correspondant
• Bordenstein Sarah R., Vanderbilt University (USA)
• Cruaud Corinne, Centre national de séquençage (FRA)
• Shaiber Alon, University of Chicago (USA)
• Esen Özcan C., University of Chicago (USA)
• Weill Mylène, ISEM (FRA)
• Makoundou Patrick, ISEM (FRA)
• Lolans Karen, University of Chicago (USA)
• Watson Andrea R., University of Chicago (USA)
• Rakotoarivony Ignace, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0009-0002-3480-6734
• Bordenstein Seth R., Vanderbilt University (USA)
• Eren A. Murat, University of Chicago (USA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598844/)

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