Specificity and genetic polymorphism in the Vfm quorum sensing system of plant pathogenic bacteria of the genus Dickeya

Hugouvieux-Cotte-Pattat Nicole, Royer Monique, Gueguen Erwan, Le Guen Paul, Süssmuth Roderich, Reverchon Sylvie, Cociancich Stéphane. 2022. Specificity and genetic polymorphism in the Vfm quorum sensing system of plant pathogenic bacteria of the genus Dickeya. Environmental Microbiology, 24 (3), n.spéc. Pathogen and Antimicrobial Resistance Ecology : 1467-1483.

Résumé : The Vfm quorum sensing (QS) system is preponderant for the virulence of different species of the bacterial genus Dickeya. The vfm gene cluster encodes 26 genes involved in the production, sensing or transduction of the QS signal. To date, the Vfm QS signal has escaped detection by analytical chemistry methods. However, we report here a strain-specific polymorphism in the biosynthesis genes vfmO and vfmP, which is predicted to be related to the production of different analogues of the QS signal. Consequently, the Vfm communication could be impossible between strains possessing different variants of the genes vfmO/P. We constructed three Vfm QS biosensor strains possessing different vfmO/P variants and compared these biosensors for their responses to samples prepared from 34 Dickeya strains possessing different vfmO/P variants. A pattern of specificity was demonstrated, providing evidence that the polymorphism in the genes vfmO/P determines the biosynthesis of different analogues of the QS signal. Unexpectedly, this vfmO/P-dependent pattern of specificity is linked to a polymorphism in the ABC transporter gene vfmG, suggesting an adaptation of the putative permease VfmG to specifically bind different analogues of the QS signal. Accordingly, we discuss the possible involvement of VfmG as co-sensor of the Vfm two-component regulatory system.

Mots-clés Agrovoc : Dickeya chrysanthemi, Enterobacteriaceae, bactérie gram négatif, gène, virulence, pouvoir pathogène, polymorphisme génétique, variant, biodétecteur, expérimentation in vitro, technique de prévision, Dickeya, processus physiologiques cellulaires

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Institut National des Sciences Appliquées de Lyon, Agence Nationale de la Recherche, Deutsche Forschungsgemeinschaft

Auteurs et affiliations

• Hugouvieux-Cotte-Pattat Nicole, INSA de Lyon (FRA)
• Royer Monique, CIRAD-BIOS-UMR PHIM (FRA)
• Gueguen Erwan, INSA de Lyon (FRA)
• Le Guen Paul, Université de Montpellier (FRA)
• Süssmuth Roderich, Technische Universitaet Berlin (DEU)
• Reverchon Sylvie, INSA de Lyon (FRA)
• Cociancich Stéphane, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-8601-3598 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600790/)

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