Genome characterization and complete sequence of a new badnavirus from Pandanus amaryllifolius

Alvarez-Quinto Robert A., Grinstead Samuel, Rott Philippe, Mollow Dimitre. 2022. Genome characterization and complete sequence of a new badnavirus from Pandanus amaryllifolius. Archives of Virology, 167 : 1717-1720.

https://doi.org/10.1007/s00705-022-05480-0

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Résumé : A new badnavirus was sequenced from fragrant pandan grass (Pandanus amaryllifolius) displaying mosaic and chlorosis on the leaves. The complete genome sequence was determined by high-throughput sequencing. The new badnavirus was tentatively named "pandanus mosaic associated virus" (PMaV). Similar to those of other members of the genus Badnavirus, the genome of PMaV consists of a circular DNA molecule of 7,481 bp with three open reading frames (ORF) potentially coding for three proteins. ORF3 encodes a polyprotein with conserved protein domains including zinc finger, trimeric dUTPase, aspartic protease, reverse transcriptase (RT), and RNase H domains. Pairwise comparisons of the highly conserved RT + RNase H region revealed the highest nucleotide (nt) sequence identity (70.71%) to taro bacilliform CH virus-Et17 (MG017324). In addition to PMaV, viral sequences corresponding to orchid fleck dichorhavirus (OFV) were detected in the same plant sample. The complete sequence of the OFV coding region shared >98% nt sequence identity with other isolates of OFV available in the GenBank database. Disease symptoms could not be attributed exclusively to PMaV or OFV, as both viruses were present in the pandan grass exhibiting mosaic and chlorosis.

Mots-clés Agrovoc : virus des végétaux, maladie des plantes, chlorose, génome, séquence nucléotidique, génomique, séquence d'arn

Mots-clés géographiques Agrovoc : États-Unis d'Amérique, Floride

Mots-clés complémentaires : Pandanus amaryllifolius, Badnavirus

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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