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Harmonised SOP for NIRS measurement on intact cassava roots and yam tubers using NIRS FOSS. High-throughput phenotyping protocols (HTPP), WP3

Alamu Emmanuel, Adesokan Michael, Maziya-Dixon Busie, Meghar Karima, Davrieux Fabrice. 2022. Harmonised SOP for NIRS measurement on intact cassava roots and yam tubers using NIRS FOSS. High-throughput phenotyping protocols (HTPP), WP3. Ibadan : RTBfoods Project-CIRAD, 13 p.

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RTBfoods_SOP_NIRS Measurement_Intact cassava & yam_Benchtop FOSS.pdf

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Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/HXFVVS / Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/H7LUYC

Résumé : The standard operating procedure for the analysis of intact fresh yam tubers and cassava roots is described in this document. The procedures expressed require minimal sample preparation and spectra data collection using near infrared spectrophotometer (NIRS). In breeding programs, breeders often cultivate many genotypes which require rapid screening as part of the characterization process for the genotypes. However, most conventional methods used for the analysis of yam tubers and cassava roots involve considerably number of processes from peeling and chopping to drying in the oven for about 72 hours; these processes are time consuming and do not qualify as high throughput methods of analysis. To this end, a standard near infrared spectrophotometric procedure for the rapid analysis of yam and cassava samples using the intact tubers/roots is described in this SOP.

Mots-clés libres : Cassava roots, Yam tubers, Near infrared spectroscopy, Oven drying, Spectra data, High throughput, Quality traits, WinISI, ISIscan

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Centro Internacional de Agricultura Tropical, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, James Hutton Institute

Projets sur financement : (FRA) Breeding RTB Products for End User Preferences

Auteurs et affiliations

  • Alamu Emmanuel, IITA (NGA)
  • Adesokan Michael, IITA (NGA)
  • Maziya-Dixon Busie, IITA (NGA)

Contributeurs et affiliations

  • Meghar Karima, CIRAD-PERSYST-UMR Qualisud (FRA) - collaborateur
  • Davrieux Fabrice, CIRAD-PERSYST-UMR Qualisud (REU) - collaborateur

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602372/)

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