Molecular epidemiology of Ralstonia pseudosolanacearum phylotype I strains in the Southwest Indian Ocean Region and their relatedness to African strains

Cellier Gilles, Nordey Thibault, Cortada Laura, Gauche Mirana, Rasoamanana Hasina, Yahiaoui Noura, Rebert Emeline, Prior Philippe, Cheron Jean-Jacques, Poussier Stéphane, Pruvost Olivier. 2023. Molecular epidemiology of Ralstonia pseudosolanacearum phylotype I strains in the Southwest Indian Ocean Region and their relatedness to African strains. Phytopathology, 113 (3) : 423-435.

Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/BYBKB3

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : The increasing requirement for developing tools enabling fine strain traceability responsible for epidemics is tightly linked with the need to understand factors shaping pathogen populations and their environmental interactions. Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is one of the most important plant diseases in tropical and subtropical regions. Sadly, little, outdated, or no information on its epidemiology is reported in the literature, although alarming outbreaks are regularly reported as disasters. A large set of phylotype I isolates (n = 2,608) was retrieved from diseased plants in fields across the Southwest Indian Ocean (SWIO) and Africa. This collection enabled further assessment of the epidemiological discriminating power of the previously published RS1-MLVA14 scheme. Thirteen markers were validated and characterized as not equally informative. Most had little infra-sequevar polymorphism, and their performance depended on the sequevar. Strong correlation was found with a previous multilocus sequence typing scheme. However, 2 to 3% of sequevars were not correctly assigned through endoglucanase gene sequence. Discriminant analysis of principal components (DAPC) revealed four groups with strong phylogenetic relatedness to sequevars 31, 33, and 18. Phylotype I-31 isolates were highly prevalent in the SWIO and Africa, but their dissemination pathways remain unclear. Tanzania and Mauritius showed the greatest diversity of RSSC strains, as the four DAPC groups were retrieved. Mauritius was the sole territory harboring a vast phylogenetic diversity and all DAPC groups. More research is still needed to understand the high prevalence of phylotype I-31 at such a large geographic scale.

Mots-clés Agrovoc : épidémiologie, Ralstonia solanacearum, phylogénie, maladie bactérienne, variation génétique, génétique des populations, facteur du milieu, maladie des plantes, flétrissement, Ralstonia

Mots-clés géographiques Agrovoc : France, Maurice, océan Indien, La Réunion, Afrique

Mots-clés libres : Bacterial pathogens, Diseases in natural plant populations, Genetics

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission, European Regional Development Fund

Agences de financement hors UE : Agropolis Fondation, Agence Nationale de la Recherche, Conseil Général de La Réunion, Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Projets sur financement : (FRA) Agricultural Sciences for sustainable Development

Auteurs et affiliations

• Cellier Gilles, ANSES-LSV (REU) - auteur correspondant
• Nordey Thibault, CIRAD-PERSYST-UPR HortSys (FRA)
• Cortada Laura, IITA (KEN)
• Gauche Mirana, Université de la Réunion (REU)
• Rasoamanana Hasina, Université de la Réunion (REU)
• Yahiaoui Noura, Université de la Réunion (REU)
• Rebert Emeline, Université de la Réunion (REU)
• Prior Philippe, INRAE (FRA)
• Cheron Jean-Jacques, CIRAD-BIOS-UMR PVBMT (REU)
• Poussier Stéphane, Université de la Réunion (REU)
• Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604480/)

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