Resistance and susceptibility to Xanthomonas phaseoli pv. manihotis in cassava: A transcriptomic comparison (or two sides of the same coin)

Ramirez Edilene, Dereeper Alexis, Bernal Adriana Jimena, Szurek Boris, López Camilo. 2020. Resistance and susceptibility to Xanthomonas phaseoli pv. manihotis in cassava: A transcriptomic comparison (or two sides of the same coin). Physiological and Molecular Plant Pathology, 112:101535, 11 p.

Quartile : Q2, Sujet : PLANT SCIENCES

Résumé : Cassava bacterial blight caused by Xanthomonas phaseoli p.v manihotis (Xpm) is a common disease of cassava (Manihot esculenta). The molecular mechanisms underlying susceptible and resistant response and host defense mechanisms are currently unknown. In this study, we performed large-scale mRNA expression profiling using an RNAseq approach to identify Cassava responses in MBRA685 variety inoculated with Xpm618 (resistant response) and Xpm318 (susceptible response) strains. Bioinformatic analysis revealed significantly differentially expressed genes (DEGs) by Xpm681 in MBRA685 with higher fold change values. Unique prevalent GO categories for Xpm681 response included “protein kinase activity” and “phosphorylation”; “amino acid biosynthetic process” for Xpm318 response and “regulation of transcription” and “transcription factor activity” in both responses. There were particular differences between the cultivars in the intensity of expression of genes with putative roles in transcriptional activity, receptor and signaling processes, secondary metabolism and photosynthesis. The differences between two interactions can be explained quantitatively, the resistant response was weaker than the susceptible response. Quantitative real-time PCR experiments confirmed the reliability of our RNAseq data.

Mots-clés Agrovoc : Manihot esculenta, expression des gènes, Xanthomonas, bioinformatique, maladie bactérienne, Transcription génique, Xanthomonas campestris phaseoli, variété, résistance aux maladies, photosynthèse, mécanisme de défense, manioc, Xanthomonas campestris manihotis, amélioration des plantes, PCR

Mots-clés libres : Cassava bacterial blight, RNAseq, Resistance responses, Susceptibility responses

Auteurs et affiliations

• Ramirez Edilene, Universidad Nacional de Colombia (COL)
• Dereeper Alexis, CIRAD-BIOS-UMR AGAP (GLP)
• Bernal Adriana Jimena, Universidad de los Andes (COL)
• Szurek Boris, IRD (FRA)
• López Camilo, Universidad Nacional de Colombia (COL) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/605397/)

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