Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.)

Dossa Komivi, Mmadi Marie Ali, Zhou Rong, Zhang Tianyuan, Su Ruqi, Zhang Yujuan, Wang Linhai, You Jun, Zhang Xiurong. 2019. Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.). International Journal of Molecular Sciences, 20 (16), n.spéc. Plant Genomics 2019:3930, 19 p.

https://doi.org/10.3390/ijms20163930

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Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q2, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Résumé : Sesame is a source of a healthy vegetable oil, attracting a growing interest worldwide. Abiotic stresses have devastating effects on sesame yield; hence, studies have been performed to understand sesame molecular responses to abiotic stresses, but the core abiotic stress-responsive genes (CARG) that the plant reuses in response to an array of environmental stresses are unknown. We performed a meta-analysis of 72 RNA-Seq datasets from drought, waterlogging, salt and osmotic stresses and identified 543 genes constantly and differentially expressed in response to all stresses, representing the sesame CARG. Weighted gene co-expression network analysis of the CARG revealed three functional modules controlled by key transcription factors. Except for salt stress, the modules were positively correlated with the abiotic stresses. Network topology of the modules showed several hub genes predicted to play prominent functions. As proof of concept, we generated over-expressing Arabidopsis lines with hub and non-hub genes. Transgenic plants performed better under drought, waterlogging, and osmotic stresses than the wild-type plants but did not tolerate the salt treatment. As expected, the hub gene was significantly more potent than the non-hub gene. Overall, we discovered several novel candidate genes, which will fuel investigations on plant responses to multiple abiotic stresses.

Mots-clés Agrovoc : expression des gènes, Sesamum indicum, génie génétique, Transcription génique, tolérance à la sécheresse, changement climatique, stress abiotique, stress osmotique, stress dû à la sécheresse, gène, tolérance au sel, Arabidopsis thaliana, résistance à la sécheresse, analyse de réseau

Mots-clés géographiques Agrovoc : Chine

Mots-clés libres : Stress marker genes, Sesame, Gene co-expression, Abiotic stress tolerance, Hub genes, Meta-analysis

Agences de financement hors UE : China Agriculture Research System, Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences

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