Physicochemical characterization of special cassava starches and their application for bio-ethanol production through no-cook technology at very high gravity

Moreno Alzate Jhon Larry, Tran Thierry, Ceballos Hernan, Nguyen Chinh-Nghia, Nguyen Tien Cuong, Zhang Xiaofei, Newby Jonathan, Dufour Dominique, Chu-Ky Son. 2024. Physicochemical characterization of special cassava starches and their application for bio-ethanol production through no-cook technology at very high gravity. Industrial Crops and Products, 219:119095, 13 p.

Résumé : Various cassava genotypes were developed with distinct starch characteristics, including amylose-free and small-granule mutations. Despite these unique traits, the ethanol production potential of these starches has not been explored. Cassava starch plays a crucial role in ethanol production, particularly in tropical countries like Colombia. This study aimed to assess the physicochemical properties of different cassava starches (including the amylose-free and small granule mutations), and to evaluate their potential for ethanol production using the Simultaneous Liquefaction, Saccharification, and Fermentation process under very high gravity (SLSF-VHG) and no-cooking conditions. Comparative analysis revealed that two double mutant starches and small-granule starch (GM4694–1) exhibited lower resistant starch content than those from wild-type and amylose-free cassava, making them more susceptible to enzymatic breakdown. The amylose content for GM4694–1 and wild-type cassava was 21.9 and 16.1 %, respectively, while the remaining samples were amylose-free. In the SLSF-VHG process, GM4694–1 demonstrated a significant ethanol yield, surpassing 16 % v/v, equivalent to 80 % of the theoretical ethanol yield within 90 hours. This suggests that the GM4694–1 genotype has the potential to produce ethanol efficiently at a temperature of 30°C. Furthermore, the solid residue obtained after the SLSF-VHG process could serve as a high-quality feed additive. This study enhances our understanding of the properties of special cassava starches and their correlation with ethanol production.

Mots-clés Agrovoc : propriété physicochimique, amidon, manioc, génotype, amylose, bioénergie, éthanol, composition chimique, mutation, propriété technologique, lisier, amidon industriel, technologie du bois

Mots-clés géographiques Agrovoc : Colombie

Mots-clés libres : Simultaneous liquefaction saccharification and fermentation (SLSF), Very high gravity (VHG), Amylose-free starch, Small granule starch

Agences de financement hors UE : Ministry of Science and Technology of Vietnam, Consortium of International Agricultural Research Centers, Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Projets sur financement : (FRA) Roots, Tubercules and Bananas

Auteurs et affiliations

• Moreno Alzate Jhon Larry, CIAT (COL)
• Tran Thierry, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-9557-3340 - auteur correspondant
• Ceballos Hernan, CIAT (COL)
• Nguyen Chinh-Nghia, HUST (VNM)
• Nguyen Tien Cuong, HUST (VNM)
• Zhang Xiaofei, CIAT (COL)
• Newby Jonathan, CIAT (COL)
• Dufour Dominique, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-7794-8671
• Chu-Ky Son, HUST (VNM) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/609948/)

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