AFM1 exposure in male balb/c mice and intervention strategies against its immuno-physiological toxicity using clay mineral and Lactic acid bacteria alone or in combination

Aloui Amina, Ben Salah-Abbès Jalila, Belgacem Hela, Dhif Haifa, Zinedine Abdellah, Riba Amar, Meile Jean-Christophe, Durand Noel, Brabet Catherine, Abbes Samir. 2024. AFM1 exposure in male balb/c mice and intervention strategies against its immuno-physiological toxicity using clay mineral and Lactic acid bacteria alone or in combination. Immunopharmacology and Immunotoxicology, 46 (2) : 199-211.

Résumé : Context: Aflatoxins are the most harmful mycotoxins that cause human and animal health concerns. Aflatoxin M1 (AFM1) is the primary hydroxylated metabolite of aflatoxin B1 and is linked to the development of hepatocellular carcinoma and immunotoxicity in humans and animals. Because of the important role of dairy products in human life, especially children, AFM1 is such a major concern to humans because of its frequent occurrence in dairy products at concentrations high enough to cause adverse effects to human and animal health. Reduced its bioavailability becomes a high priority in order to protect human and animal health. Objectives: This study aimed to investigate, in vivo, the ability of lactic acid bacteria (lactobacillus rhamnosus GAF01, LR) and clay mineral (bentonite, BT) mixture to mitigate/reduce AFM1-induced immunotoxicity, hepatotoxicity, nephrotoxicity and oxidative stress in exposed Balb/c mice. Materials and methods: The in vivo study was conducted using male Balb/c mice that treated, orally, by AFM1 alone or in combination with LR and/or BT, daily for 10 days as follows: group 1 control received 200 µl of PBS, group 2 treated with LR alone (2.108 CFU/mL), group 3 treated with BT alone (1 g/kg bw), group 4 treated with AFM1 alone (100 μg/kg), group 5 co-treated with LR + AFM1, group 6 co-treated with BT + AFM1, group 7 co-treated with BT + LR + AFM1. Forty-eight h after the end of the treatment, the mice were sacrificed and the blood, spleen, thymus, liver and kidney were collected. The blood was used for biochemical and immunological study. Spleen and thymus samples were used to thymocytes and splenocytes assessments. Liver and kidney samples were the target for evaluation of oxidative stress enzymes status and for histological assays. Results: The results showed that AFM1 caused toxicities in male Blab/c mice at different levels. Treatment with AFM1 resulted in severe stress of liver and kidney organs indicated by a significant change in the biochemical and immunological parameters, histopathology as well as a disorder in the profile of oxidative stress enzymes levels. Also, it was demonstrated that AFM1 caused toxicities in thymus and spleen organs. The co-treatment with LR and/or BT significantly improved the hepatic and renal tissues, regulated antioxidant enzyme activities, spleen and thymus viability and biochemical and immunological parameters. LR and BT alone showed to be safe during the treatment. Conclusion: In summary, the LR and/or BT was able to reduce the biochemical, histopathological and immunological damages induced by AFM1 and indeed it could be exploited as one of the biological strategies for food and feedstuffs detoxification.

Mots-clés Agrovoc : mycotoxine, bactérie lactique, oxydation biochimique, aflatoxine, immunologie, bentonite, souris, toxicité, minéral toxique, stress oxydatif, capacité d'échange ionique

Mots-clés géographiques Agrovoc : Tunisie

Mots-clés libres : Aflatoxin M1, Bentonite, Lactic acid bacteria, Toxicity, Detoxification

Agences de financement hors UE : Tunisian Ministry of Higher Education and Scientific Research

Projets sur financement : (MAR) PHC Maghreb

Auteurs et affiliations

• Aloui Amina, CIRAD-PERSYST-UMR Qualisud (FRA)
• Ben Salah-Abbès Jalila, University of Monastir (TUN)
• Belgacem Hela, University of Monastir (TUN)
• Dhif Haifa, University of Monastir (TUN)
• Zinedine Abdellah, Chouaib Doukkali University (MAR)
• Riba Amar, Ecole Normale Supérieure de Kouba (DZA)
• Meile Jean-Christophe, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-6747-2104
• Durand Noel, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-1627-6848
• Brabet Catherine, CIRAD-PERSYST-UMR Qualisud (REU)
• Abbes Samir, CIRAD-PERSYST-UMR Qualisud (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/610300/)

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