Spatial scale influences the distribution of viral diversity in the eukaryotic virome of the mosquito Culex pipiens

Gil Patricia, Exbrayat Antoni, Loire Etienne, Rakotoarivony Ignace, Charriat Florian, Morel Come, Baldet Thierry, Boisseau Michel, Marie Albane, Francés Benoit, L'Ambert Grégory, Bessat Mohamed, Otify Yehia, Goffredo Maria, Mancini Giuseppe, Busquets Núria, Birnberg Lotty, Talavera Sandra, Aranda Carles, Ayari Emna, Mejri Selma, Sghaier Soufien, Bennouna Amal, El Rhaffouli Hicham, Balenghien Thomas, Chlyeh Ghita, Fassi Fihri Ouafaa, Reveillaud Julie, Simonin Yannick, Eloit Marc, Gutierrez Serafin. 2023. Spatial scale influences the distribution of viral diversity in the eukaryotic virome of the mosquito Culex pipiens. Virus Evolution, 9 (2):vead054, 14 p.

Résumé : Our knowledge of the diversity of eukaryotic viruses has recently undergone a massive expansion. This diversity could influence host physiology through yet unknown phenomena of potential interest to the fields of health and food production. However, the assembly processes of this diversity remain elusive in the eukaryotic viromes of terrestrial animals. This situation hinders hypothesis-driven tests of virome influence on host physiology. Here, we compare taxonomic diversity between different spatial scales in the eukaryotic virome of the mosquito Culex pipiens. This mosquito is a vector of human pathogens worldwide. The experimental design involved sampling in five countries in Africa and Europe around the Mediterranean Sea and large mosquito numbers to ensure a thorough exploration of virus diversity. A group of viruses was found in all countries. This core group represented a relatively large and diverse fraction of the virome. However, certain core viruses were not shared by all host individuals in a given country, and their infection rates fluctuated between countries and years. Moreover, the distribution of coinfections in individual mosquitoes suggested random co-occurrence of those core viruses. Our results also suggested differences in viromes depending on geography, with viromes tending to cluster depending on the continent. Thus, our results unveil that the overlap in taxonomic diversity can decrease with spatial scale in the eukaryotic virome of C. pipiens. Furthermore, our results show that integrating contrasted spatial scales allows us to identify assembly patterns in the mosquito virome. Such patterns can guide future studies of virome influence on mosquito physiology.

Mots-clés Agrovoc : vecteur de maladie, virologie, Culex pipiens

Mots-clés complémentaires : virome, Métagénomique

Mots-clés libres : Virome, Mosquito, Culex pipiens, Metagenomics

Classification Agris : L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Agences de financement hors UE : Montpellier Université d'Excellence, Direction générale de l'alimentation

Programme de financement européen : FP7

Projets sur financement : (EU) Emerging viral vector borne diseases, (FRA) ArboSud

Auteurs et affiliations

• Gil Patricia, CIRAD-BIOS-UMR ASTRE (FRA)
• Exbrayat Antoni, CIRAD-BIOS-UMR ASTRE (FRA)
• Loire Etienne, CIRAD-BIOS-UMR ASTRE (FRA)
• Rakotoarivony Ignace, CIRAD-BIOS-UMR ASTRE (FRA)
• Charriat Florian, CIRAD-BIOS-UMR ASTRE (FRA)
• Morel Come, CIRAD-BIOS-UMR ASTRE (FRA)
• Baldet Thierry, CIRAD-BIOS-UMR ASTRE (REU) ORCID: 0000-0003-2979-9517
• Boisseau Michel, INRAE (FRA)
• Marie Albane, EID (FRA)
• Francés Benoit, EID (FRA)
• L'Ambert Grégory, EID (FRA)
• Bessat Mohamed, Alexandria University (EGY)
• Otify Yehia, Alexandria University (EGY)
• Goffredo Maria, IZSA&M (ITA)
• Mancini Giuseppe, IZSA&M (ITA)
• Busquets Núria, CReSA (ESP)
• Birnberg Lotty, CReSA (ESP)
• Talavera Sandra, CReSA (ESP)
• Aranda Carles, CReSA (ESP)
• Ayari Emna, IRVT (TUN)
• Mejri Selma, IRVT (TUN)
• Sghaier Soufien, IRVT (TUN)
• Bennouna Amal, IAV Hassan II (MAR)
• El Rhaffouli Hicham, FAR Military Health Service (MAR)
• Balenghien Thomas, CIRAD-BIOS-UMR ASTRE (FRA)
• Chlyeh Ghita, IAV Hassan II (MAR)
• Fassi Fihri Ouafaa, IAV Hassan II (MAR)
• Reveillaud Julie, INRAE (FRA)
• Simonin Yannick, CIRAD-BIOS-UMR ASTRE (FRA)
• Eloit Marc, Institut Pasteur (FRA)
• Gutierrez Serafin, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-5277-7239 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/606675/)

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