Plant lipases: Biocatalyst aqueous environment in relation to optimal catalytic activity in lipase-catalyzed synthesis reactions

Caro Yanis, Pina Michel, Turon Fabrice, Guilbert Stéphane, Mougeot Estelle, Fetsch David V., Attwool Philip, Graille Jean. 2002. Plant lipases: Biocatalyst aqueous environment in relation to optimal catalytic activity in lipase-catalyzed synthesis reactions. Biotechnology and Bioengineering, 77 (6) : 693-703.

https://doi.org/10.1002/bit.10155

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
515358.pdf
Télécharger (273kB)

Résumé : Adsorption and desorption isotherms of two commercial enzyme preparations of papain and bromelain were determined with a Dynamic Vapor System. The Guggenheim-Anderson-deBoer (GAB) modeling of the obtained sorption isotherms allowed the definition of different levels of hydration of those samples. Afterward, these enzyme preparations were used as biocatalysts in water and solvent-free esterification and alcoholysis reactions. The evolution of the obtained fatty acid ester level as a function of the initial hydration level of the biocatalyst, i.e., thermodynamic water activity (aw) and water content, was studied. The results show an important correlation between the initial hydration level of the biocatalyst and its catalytic activity during the lipase-catalyzed synthesis reactions. Thus, the Carica papaya lipase (crude papain preparation) catalytic activity is highly dependent on the biocatalyst hydration state. The optimized synthesis reaction yield is obtained when the a. value of the enzyme preparation is stabilized at 0.22, which corresponds to 2% water content. This optimal level of hydration occurs on the linear part of the biocatalyst's sorption isotherm, where the water molecules can form a mono- or multiple layer with the protein network. The synthesis reaction yield decreases when the aw of the preparation is higher than 0.22, because the excess water molecules modify the system equilibrium leading to the reverse and competitive reaction, i.e., hydrolysis. These results show also that an optimal storage condition for the highly hydrophilic crude papain preparation is a relative humidity strictly lower than 70% to avoid an irreversible structural transition leading to a useless biocatalyst. Concerning the bromelain preparation, no effect of the hydration level on the catalytic activity during esterification reactions was observed. This biocatalyst has too weak a catalytic activity which makes it difficult to observe any differences. Furthermore, the bromelain preparation is far more hydrophobic as it adsorbs only 18 g of water per 100 g of dry material at aw around 0.90. No deliquescence of thi's enzymatic preparation is observed at this aw value.

Mots-clés Agrovoc : estérase, biocatalyseur, plante, Carica papaya, papaine, estérification, Activité de l'eau, activité enzymatique, sorption, lipase, thermodynamique