Identification and characterisation of a highly divergent geminivirus: Evolutionary and taxonomic implications

Bernardo Pauline, Golden Michael, Akram Mohammad, Naimuddin, Nadarajan Nagaswamy, Fernandez Emmanuel, Granier Martine, Rebelo Anthony, Peterschmitt Michel, Martin Darren Patrick, Roumagnac Philippe. 2013. Identification and characterisation of a highly divergent geminivirus: Evolutionary and taxonomic implications. Virus Research, 177 (1) : 35-45.

Quartile : Q3, Sujet : VIROLOGY

Résumé : During a large scale "non a priori" survey in 2010 of South African plant-infecting single stranded DNA viruses, a highly divergent geminivirus genome was isolated from a wild spurge, Euphorbia caputmedusae. In addition to being infectious in E. caput-medusae, the cloned viral genome was also infectious in tomato and Nicotiana benthamiana. The virus, named Euphorbia caput-medusae latent virus (EcmLV) due to the absence of infection symptoms displayed by its natural host, caused severe symptoms in both tomato and N. benthamiana. The genome organisation of EcmLV is unique amongst geminiviruses and it likely expresses at least two proteins without any detectable homologues within public sequence databases. Although clearly a geminivirus, EcmLV is so divergent that we propose its placement within a new genus that we have tentatively named Capulavirus. Using a set of highly divergent geminiviruses genomes, it is apparent that recombination has likely been a primary process in the genus-level diversification of geminiviruses. It is also demonstrated how this insight, taken together with phylogenetic analyses of predicted coat protein and replication associated protein (Rep) amino acid sequences indicate that the most recent common ancestor of the geminiviruses was likely a dicot-infecting virus that, like modern day mastreviruses and becurtoviruses, expressed its Rep from a spliced complementary strand transcript.

Mots-clés Agrovoc : virus des végétaux, espèce nouvelle, Geminiviridae, identification, Euphorbia, Solanum lycopersicum, Nicotiana, génome, taxonomie, phylogénie, évolution, adn, virulence, séquence d'adn, plante hôte

Mots-clés géographiques Agrovoc : Afrique du Sud, France

Mots-clés complémentaires : Euphorbia caputmedusae

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Bernardo Pauline, CIRAD-BIOS-UMR BGPI (FRA)
• Golden Michael, Institute of Infectious Diseases and Molecular Medicine (ZAF)
• Akram Mohammad, Indian Institute of Pulses Research (IND)
• Naimuddin, Indian Institute of Pulses Research (IND)
• Nadarajan Nagaswamy, Indian Institute of Pulses Research (IND)
• Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
• Granier Martine, CIRAD-BIOS-UMR BGPI (FRA)
• Rebelo Anthony, SANBI (ZAF)
• Peterschmitt Michel, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-8709-9418
• Martin Darren Patrick, Institute of Infectious Diseases and Molecular Medicine (ZAF)
• Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039

Source : Cirad - Agritrop (https://agritrop.cirad.fr/571161/)

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