In Silico analysis of usher encoding genes in Klebsiella pneumoniae and characterization of their role in adhesion and colonization

Khater Fida, Balestrino Damien, Charbonnel Nicolas, Dufayard Jean-François, Brisse Sylvain, Forestier Christianne. 2015. In Silico analysis of usher encoding genes in Klebsiella pneumoniae and characterization of their role in adhesion and colonization. PloS One, 10 (3):e0116215, 24 p.

https://doi.org/10.1371/journal.pone.0116215

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Url - jeu de données - Entrepôt autre : http://www.ebi.ac.uk/ena/data/view/PRJEB7075 / Url - jeu de données - Entrepôt autre : http://www.ebi.ac.uk/ena/data/view/ERS537769 / Url - jeu de données - Entrepôt autre : http://www.ebi.ac.uk/ena/data/view/CCVM01000001

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Note générale : Jeux de données enregistrés dans la base ENA du numéro d'accès CCVM01000001 au CCVM01000154.

Résumé : Chaperone/usher (CU) assembly pathway is used by a wide range of Enterobacteriaceae to assemble adhesive surface structures called pili or fimbriae that play a role in bacteria-host cell interactions. In silico analysis revealed that the genome of Klebsiella pneumoniae LM21 harbors eight chromosomal CU loci belonging to to γκп and ϭ clusters. Of these, only two correspond to previously described operons, namely type 1 and type 3-encoding operons. Isogenic usher deletion mutants of K. pneumoniae LM21 were constructed for each locus and their role in adhesion to animal (Intestine 407) and plant (Arabidopsis thaliana) cells, biofilm formation and murine intestinal colonization was investigated. Type 3 pili usher deleted mutant was impaired in all assays, whereas type 1 pili usher deleted mutant only showed attenuation in adhesion to plant cells and in intestinal colonization. The The LM21ΔkpjC mutant was impaired in its capacity to adhere to Arabidopsis cells and to colonize the murine intestine, either alone or in co-inoculation experiments. Deletion of LM21ΔkpjC induced a significant decrease in biofilm formation, in adhesion to animal cells and in colonization of the mice intestine. The LM21ΔkpaC and LM21ΔkpeC mutants were only attenuated in biofilm formation and the adhesion abilities to Arabidopsis cells, respectively. No clear in vitro or in vivo effect was observed for LM21ΔkpbC and LM21ΔkpdC mutants. The multiplicity of CU loci in K. pneumoniae genome and their specific adhesion pattern probably reflect the ability of the bacteria to adhere to different substrates in its diverse ecological niches.

Mots-clés Agrovoc : Klebsiella pneumoniae, gène, code génétique, génome, phylogénie, mutagène, Enterobacteriaceae, Arabidopsis thaliana, infestation, adsorption, adhésivité