A large-scale genomic approach affords unprecedented resolution for the molecular epidemiology and evolutionary history of contagious caprine pleuropneumonia

Dupuy Virginie, Verdier Axel, Thiaucourt François, Manso-Silvan Lucia. 2015. A large-scale genomic approach affords unprecedented resolution for the molecular epidemiology and evolutionary history of contagious caprine pleuropneumonia. Veterinary Research, 46 (74), 11 p.

Quartile : Q1, Sujet : VETERINARY SCIENCES

Résumé : Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp), is a devastating disease of domestic goats and of some wild ungulate species. The disease is currently spreading in Africa and Asia and poses a serious threat to disease-free areas. A comprehensive view of the evolutionary history and dynamics of Mccp is essential to understand the epidemiology of CCPP. Yet, analysing the diversity of genetically monomorphic pathogens, such as Mccp, is complicated due to their low variability. In this study, the molecular epidemiology and evolution of CCPP was investigated using a large-scale genomic approach based on next-generation sequencing technologies, applied to a sample of strains representing the global distribution of this disease. A highly discriminatory multigene typing system was developed, allowing the differentiation of 24 haplotypes among 25 Mccp strains distributed in six genotyping groups, which showed some correlation with geographic origin. A Bayesian approach was used to infer the first robust phylogeny of the species and to date the principal events of its evolutionary history. The emergence of Mccp was estimated only at about 270 years ago, which explains the low genetic diversity of this species despite its high mutation rate, evaluated at 1.3 × 10 −6 substitutions per site per year. Finally, plausible scenarios were proposed to elucidate the evolution and dynamics of CCPP in Asia and Africa, though limited by the paucity of Mccp strains, particularly in Asia. This study shows how combining large-scale genomic data with spatial and temporal data makes it possible to obtain a comprehensive view of the epidemiology of CCPP, a precondition for the development of improved disease surveillance and control measures.

Mots-clés Agrovoc : phylogénie, Mycoplasma capricolum, épidémiologie, pleuropneumonie contagieuse caprine, variation génétique, évolution, distribution géographique, séquence nucléotidique, marqueur génétique, génome, maladie des animaux, caprin, génotype, surveillance épidémiologique

Mots-clés géographiques Agrovoc : Afrique

Mots-clés libres : Contagious caprine pleuropneumonia, CCPP, Mycoplasma capricolum subsp. capripneumoniae, Mccp, Genotyping, Molecular epidemiology, Evolutionary history, Large-scale genomic approach

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Dupuy Virginie, CIRAD-BIOS-UMR CMAEE (FRA)
• Verdier Axel, INRA (FRA)
• Thiaucourt François, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Manso-Silvan Lucia, CIRAD-BIOS-UPR Contrôle des maladies (FRA) ORCID: 0000-0002-5539-3804

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578842/)

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