A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)

Jaramillo Angélica M., Londoño Luis Fernando, Orozco Juan Camilo, Patiño Gelver, Belalcazar John, Davrieux Fabrice, Talsma Elise F.. 2018. A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta). PloS One, 13 (12):e0209702, 14 p.

Quartile : Q2, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment.

Mots-clés Agrovoc : Manihot esculenta, composition chimique, carotène, spectroscopie infrarouge

Classification Agris : Q04 - Composition des produits alimentaires
Q01 - Sciences et technologies alimentaires - Considérations générales
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 3 (2014-2018) - Alimentation durable

Auteurs et affiliations

• Jaramillo Angélica M., CIAT (COL)
• Londoño Luis Fernando, CIAT (COL)
• Orozco Juan Camilo, CIAT (COL)
• Patiño Gelver, CIAT (COL)
• Belalcazar John, CIAT (COL)
• Davrieux Fabrice, CIRAD-PERSYST-UMR Qualisud (REU)
• Talsma Elise F., CIAT (COL) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591121/)

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