Hennechart-Collette Catherine, Gonzalez Gaëlle, Fourniol Lisa, Fraisse Audrey, Beck Cécile, Moutailler Sara, Bournez Laure, Dheilly Nolwenn M., Lacour Sandrine A., Lecollinet Sylvie, Martin-Latil Sandra, Perelle Sylvie. 2022. Method for tick-borne encephalitis virus detection in raw milk products. Food Microbiology, 104:104003, 6 p.
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Résumé : The transmission of tick-borne encephalitis virus (TBEV) through food is rare, but can occur through the consumption of raw milk products from animals infected by tick bites. In 2020, France faced a TBEV outbreak linked to the consumption of unpasteurized goat cheese. The aim of this study was to develop and characterize a molecular method for the detection of TBEV in raw milk products based on the recent international standard PR ISO/DIS 16140-4. The TBEV recovery rates varied with the inoculation level and settings. The LOD50 and LOD95 of TBEV were 6.40 × 103 genome copies per g or per mL and 2.84 × 104 genome copies per g or per mL, respectively. The percentages of RT-qPCR inhibitions were lower than 75% and the murine norovirus (MNV-1), used as process control, was detected in all samples with a recovery rate higher than 1%, as recommended in ISO 15216. We conclude that the described method is appropriate to detect TBEV in raw milk products for routine diagnosis, and to assess potential health risks.
Mots-clés Agrovoc : encéphalite à tiques, flavivirus, lait cru, produit alimentaire, sécurité sanitaire des aliments, contamination des aliments, alimentation humaine, aliment d'origine animale, contrôle de qualité alimentaire, PCR, conformité, réglementation, procédure, mode opératoire normalisé
Mots-clés géographiques Agrovoc : France
Mots-clés complémentaires : Tick-Borne Encephalitis Virus (TBEV)
Mots-clés libres : Tick-borne encephalitis virus, Raw dairy products, RT-qPCR detection, Process control
Classification Agris : Q03 - Contamination et toxicologie alimentaires
Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes
Auteurs et affiliations
- Hennechart-Collette Catherine, Université Paris-Est Créteil Val-de-Marne (FRA)
- Gonzalez Gaëlle, INRAE (FRA)
- Fourniol Lisa, INRAE (FRA)
- Fraisse Audrey, Université Paris-Est Créteil Val-de-Marne (FRA)
- Beck Cécile, ANSES (FRA)
- Moutailler Sara, ANSES (FRA)
- Bournez Laure, ANSES (FRA)
- Dheilly Nolwenn M., INRAE (FRA)
- Lacour Sandrine A., INRAE (FRA)
-
Lecollinet Sylvie, CIRAD-BIOS-UMR ASTRE (GLP)
ORCID: 0000-0001-8535-2133
- Martin-Latil Sandra, Université Paris-Est Créteil Val-de-Marne (FRA)
- Perelle Sylvie, Université Paris-Est Créteil Val-de-Marne (FRA) - auteur correspondant
Source : Cirad-Agritrop (https://agritrop.cirad.fr/601569/)
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