Spodoptera littoralis genome mining brings insights on the dynamic of expansion of gustatory receptors in polyphagous noctuidae

Meslin Camille, Mainet Pauline, Montagné Nicolas, Robin Stéphanie, Legeai Fabrice, Bretaudeau Anthony, Johnston J Spencer, Koutroumpa Fotini, Persyn Emma, Monsempès Christelle, François Marie-Christine, Jacquin-Jolyb Emmanuelle. 2022. Spodoptera littoralis genome mining brings insights on the dynamic of expansion of gustatory receptors in polyphagous noctuidae. G3 - Genes Genomes Genetics, 12 (8):jkac131, 12 p.

Url - jeu de données - Entrepôt autre : https://data.inrae.fr/dataverse/slittoralis_genome / Url - autres données associées : https://bipaa.genouest.org/

Résumé : The bitter taste, triggered via gustatory receptors, serves as an important natural defense against the ingestion of poisonous foods in animals, and the increased host breadth is usually linked to an increase in the number of gustatory receptor genes. This has been especially observed in polyphagous insect species, such as noctuid species from the Spodoptera genus. However, the dynamic and physical mechanisms leading to these gene expansions and the evolutionary pressures behind them remain elusive. Among major drivers of genome dynamics are the transposable elements but, surprisingly, their potential role in insect gustatory receptor expansion has not been considered yet. In this work, we hypothesized that transposable elements and possibly positive selection would be involved in the highly dynamic evolution of gustatory receptor in Spodoptera spp. We first sequenced de novo the full 465 Mb genome of S. littoralis, and manually annotated the main chemosensory genes, including a large repertoire of 373 gustatory receptor genes (including 19 pseudogenes). We also improved the completeness of S. frugiperda and S. litura gustatory receptor gene repertoires. Then, we annotated transposable elements and revealed that a particular category of class I retrotransposons, the SINE transposons, was significantly enriched in the vicinity of gustatory receptor gene clusters, suggesting a transposon-mediated mechanism for the formation of these clusters. Selection pressure analyses indicated that positive selection within the gustatory receptor gene family is cryptic, only 7 receptors being identified as positively selected. Altogether, our data provide a new good quality Spodoptera genome, pinpoint interesting gustatory receptor candidates for further functional studies and bring valuable genomic information on the mechanisms of gustatory receptor expansions in polyphagous insect species.

Mots-clés Agrovoc : phylogénie, génome, séquence nucléotidique, Noctuidae, Spodoptera littoralis, intoxication alimentaire, Spodoptera litura, transposon

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Spodoptera littoralis, Gustatory receptors, Transposable elements

Agences de financement européennes : European Commission

Agences de financement hors UE : Agence Nationale de la Recherche

Programme de financement européen : H2020

Projets sur financement : (FRA) Bio-olfacticides: produire plus avec moins d'insecticides, (FRA) Evolution de la communication phéromonale chez les papillons du genre Spodoptera, (EU) IGNITE - Comparative genomics of non-model invertebrates Programme

Auteurs et affiliations

• Meslin Camille, INRAE (FRA)
• Mainet Pauline, INRAE (FRA)
• Montagné Nicolas, INRAE (FRA)
• Robin Stéphanie, INRAE (FRA)
• Legeai Fabrice, INRAE (FRA)
• Bretaudeau Anthony, INRAE (FRA)
• Johnston J Spencer, Texas A&M University (USA)
• Koutroumpa Fotini, INRAE (FRA)
• Persyn Emma, CIRAD-BIOS-UMR PVBMT (REU)
• Monsempès Christelle, INRAE (FRA)
• François Marie-Christine, INRAE (FRA)
• Jacquin-Jolyb Emmanuelle, INRAE (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/608003/)

Métriques

[ Page générée et mise en cache le 2024-06-02 ]