Cyanobacteria as source of marine bioactive compounds: Molecular specific detection based on Δ9 desaturase gene

Ben Amor Faten, Barkallah Mohamed, Elleuch Fatma, Karkouch Nesrine, Dammak Mouna, Baréa Bruno, Villeneuve Pierre, Abdelkafi Slim, Fendri Imen. 2017. Cyanobacteria as source of marine bioactive compounds: Molecular specific detection based on Δ9 desaturase gene. International Journal of Biological Macromolecules, 105 (Part 2) : 1440-1445.

https://doi.org/10.1016/j.ijbiomac.2017.07.139

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Quartile : Q1, Sujet : POLYMER SCIENCE / Quartile : Q1, Sujet : CHEMISTRY, APPLIED / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : The blue-green microalga, Arthrospira sp., isolated from the sea of Kssour Essef in Mahdia (Tunisia), was purified and then identified both morphologically and genetically based on 16S rRNA gene sequence. Following physicochemical analysis, the prokaryotic microalga tested represented a competitive source of pigments and showed a considerable rate in protein (64%) which was confirmed by FTIR measurement. The lipid content (4%) was quantified by the gravimetric method and the intracellular lipid bodies were detected with the Nile red staining. Using gas chromatography coupled with flame ionization detector, the fatty acid profile revealed the presence of 27.4% and 32.88% of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), respectively. Given the richness of the isolated microalga in unsaturated fatty acids, we have developed a SYBR Green real time PCR method for the specific identification of Arthrospira sp. Δ9 desaturase gene. This current method will be of great value for carrying out high-throughput studies like cloning, heterologous expression and structure-function relationship analysis.

Mots-clés Agrovoc : acide gras, acide gras insaturé, séquence nucléotidique, microalgue, identification, PCR, gène, chromatographie en phase gazeuse, génétique moléculaire

Mots-clés géographiques Agrovoc : Tunisie

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes
M12 - Production de l'aquaculture

Champ stratégique Cirad : Hors axes (2014-2018)

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