Rapid determination of polyphenols and vitamin C in plant-derived products

Georgé Stéphane, Brat Pierre, Alter Pascaline, Amiot Marie Josèphe. 2005. Rapid determination of polyphenols and vitamin C in plant-derived products. Journal of Agricultural and Food Chemistry, 53 (5) : 1370-1373.

Résumé : Polyphenols, widely spread in our diet by the consumption of plant food products, are commonly determined using Folin-Ciocalteu reagent that interacts with other different reducing nonphenolic substances and leads to an overestimation of polyphenol content. In this paper we report an optimized Folin-Ciocalteu method to specifically determine the contents of total polyphenols and vitamin C. After the optimal conditions for the colorimetric assay were set, solid-phase extraction (Oasis HLB (hydrophilic-lipophilic balance)) was carried out to eliminate the water-soluble reducing interferences including vitamin C. Colorimetric correction was thus performed by subtracting interfering substances contained in the water washing extract from the raw extract. Moreover, vitamin C present in the water washing extract can be destroyed by heating and thus colorimetrically deduced. This procedure was set up with synthetic solutions and validated on different extracts from fruit products.

Mots-clés Agrovoc : fruits, pulpe de fruits, polyphénol, acide ascorbique, colorimétrie, purification, teneur en phénols, teneur en vitamines, méthode

Classification Agris : Q02 - Traitement et conservation des produits alimentaires
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

• Georgé Stéphane, CTCPA (FRA)
• Brat Pierre, CIRAD-FLHOR-UPR Qualité des aliments (FRA) ORCID: 0000-0003-0429-9575
• Alter Pascaline, CIRAD-FLHOR-UPR Qualité des aliments (FRA)
• Amiot Marie Josèphe, INSERM (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525245/)

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