Evaluation of Rhizopus oryzae lipase for the determination of regiodistribution in triacylglycerols with medium chain fatty acids

Perignon Marlène, Lecomte Jérôme, Pina Michel, Renault Anne, Simmoneau-Deve Camille, Villeneuve Pierre. 2012. Evaluation of Rhizopus oryzae lipase for the determination of regiodistribution in triacylglycerols with medium chain fatty acids. Journal of the American Oil Chemists' Society, 89 (1) : 89-96.

https://doi.org/10.1007/s11746-011-1884-y

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Quartile : Q2, Sujet : CHEMISTRY, APPLIED / Quartile : Q2, Sujet : FOOD SCIENCE & TECHNOLOGY

Résumé : The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eightcarbon- chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids.

Mots-clés Agrovoc : Rhizopus oryzae, activité enzymatique, acide gras, hydrolyse enzymatique, triglycéride, estérase, lipase

Classification Agris : 000 - Autres thèmes
Q02 - Traitement et conservation des produits alimentaires
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

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