Metagenomic-based screening and molecular characterization of cowpea-infecting viruses in Burkina Faso

Palanga Essowé, Filloux Denis, Martin Darren Patrick, Fernandez Emmanuel, Gargani Daniel, Ferdinand Romain, Zabré Jean, Bouda Zakaria, Neya Bouma, Sawadogo Mahamadou, Traoré Oumar, Peterschmitt Michel, Roumagnac Philippe. 2016. Metagenomic-based screening and molecular characterization of cowpea-infecting viruses in Burkina Faso. PloS One, 11 (10):e0165188, 21 p.

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Cowpea, (Vigna unguiculata L. (Walp)) is an annual tropical grain legume. Often referred to as “poor man's meat”, cowpea is one of the most important subsistence legumes cultivated in West Africa due to the high protein content of its seeds. However, African cowpea production can be seriously constrained by viral diseases that reduce yields. While twelve cowpea-infecting viruses have been reported from Africa, only three of these have so-far been reported from Burkina Faso. Here we use a virion-associated nucleic acids (VANA)-based metagenomics method to screen for the presence of cowpea viruses from plants collected from the three agro-climatic zones of Burkina Faso. Besides the three cowpea-infecting virus species which have previously been reported from Burkina Faso (Cowpea aphid borne mosaic virus [Family Potyviridae], the Blackeye cowpea mosaic virus—a strain of Bean common mosaic virus—[Family Potyviridae] and Cowpea mottle virus [Family Tombusviridae]) five additional viruses were identified: Southern cowpea mosaic virus (Sobemovirus genus), two previously uncharacterised polerovirus-like species (Family Luteoviridae), a previously uncharacterised tombusvirus-like species (Family Tombusviridae) and a previously uncharacterised mycotymovirus-like species (Family Tymoviridae). Overall, potyviruses were the most prevalent cowpea viruses (detected in 65.5% of samples) and the Southern Sudan zone of Burkina Faso was found to harbour the greatest degrees of viral diversity and viral prevalence. Partial genome sequences of the two novel polerovirus-like and tombusvirus-like species were determined and RT-PCR primers were designed for use in Burkina Faso to routinely detect all of these cowpea-associated viruses.

Mots-clés Agrovoc : Vigna unguiculata, virologie, virus des végétaux, identification, biologie moléculaire, génie génétique, espèce nouvelle, PCR, phylogénie, séquence nucléotidique, distribution géographique, génétique des populations, contrôle de maladies

Mots-clés géographiques Agrovoc : Burkina Faso

Mots-clés complémentaires : Séquencage

Classification Agris : H20 - Maladies des plantes
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Palanga Essowé, CIRAD-BIOS-UMR BGPI (FRA)
• Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
• Martin Darren Patrick, Institute of Infectious Diseases and Molecular Medicine (ZAF)
• Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
• Gargani Daniel, CIRAD-BIOS-UMR BGPI (FRA)
• Ferdinand Romain, CIRAD-BIOS-UMR BGPI (FRA)
• Zabré Jean, INERA (BFA)
• Bouda Zakaria, INERA (BFA)
• Neya Bouma, INERA (BFA)
• Sawadogo Mahamadou, INERA (BFA)
• Traoré Oumar, INERA (BFA)
• Peterschmitt Michel, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-8709-9418
• Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582004/)

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