Evaluation of an alternative spectroscopic approach for aflatoxin analysis: Comparative analysis of food and feed samples with UPLC–MS/MS

Campbell Katrina, Ferreira Cavalcante Ana L., Galvin-King Pamela, Oplatowska-Stachowiak Michalina, Brabet Catherine, Metayer Isabelle, Montet Didier, Haughey Simon A., Elliott Christopher T.. 2017. Evaluation of an alternative spectroscopic approach for aflatoxin analysis: Comparative analysis of food and feed samples with UPLC–MS/MS. Sensors and Actuators B. Chemical, 239 : 1087-1097.

Quartile : Outlier, Sujet : INSTRUMENTS & INSTRUMENTATION / Quartile : Outlier, Sujet : CHEMISTRY, ANALYTICAL / Quartile : Q1, Sujet : ELECTROCHEMISTRY

Résumé : Increasing research has highlighted the effects of changing climates on the occurrence and prevalence of toxigenic Aspergillus species producing aflatoxins. There is concern of the toxicological effects to human health and animal productivity following acute and chronic exposure that may affect the future ability to provide safe and sufficient food globally. Considerable research has focused on the detection of these toxins, based on the physicochemical and biochemical properties of the aflatoxin compounds, in agricultural products for human and animal consumption. As improvements in food security continue more regulations for acceptable levels of aflatoxins have arisen globally; the most stringent in Europe. These regulations are important for developing countries as aflatoxin occurrence is high significantly effecting international trade and the economy. In developed countries analytical approaches have become highly sophisticated, capable of attaining results with high precision and accuracy, suitable for regulatory laboratories. Regrettably, many countries that are affected by aflatoxin contamination do not have resources for high tech HPLC and MS instrumentation and require more affordable, yet robust equally accurate alternatives that may be used by producers, processors and traders in emerging economies. It is especially important that those companies wishing to exploit the opportunities offered by lucrative but highly regulated markets in the developed world, have access to analytical methods that will ensure that their exports meet their customers quality and safety requirements. This work evaluates the ToxiMet system as an alternative approach to UPLC–MS/MS for the detection and determination of aflatoxins relative to current European regulatory standards. Four commodities: rice grain, maize cracked and flour, peanut paste and dried distillers grains were analysed for natural aflatoxin contamination. For B1 and total aflatoxins determination the qualitative correlation, above or below the regulatory limit, was good for all commodities with the exception of the dried distillers grain samples for B1 for which no calibration existed. For B1 the quantitative R2 correlations were 0.92, 0.92, 0.88 (<250 μg/kg) and 0.7 for rice, maize, peanuts and dried distillers grain samples respectively whereas for total aflatoxins the quantitative correlation was 0.92, 0.94, 0.88 and 0.91. The ToxiMet system could be used as an alternative for aflatoxin analysis for current legislation but some consideration should be given to aflatoxin M1 regulatory levels for these commodities considering the high levels detected in this study especially for maize and peanuts.

Mots-clés Agrovoc : aflatoxine, produit alimentaire, aliment pour animaux, technique analytique, identification, spectroscopie, maïs, riz, arachide, farine de maïs, drêche de brasserie, contamination biologique, réglementation

Mots-clés géographiques Agrovoc : Pays de l'Union européenne

Classification Agris : Q03 - Contamination et toxicologie alimentaires
Q53 - Contamination et toxicologie des aliments pour animaux
U30 - Méthodes de recherche
D50 - Législation

Champ stratégique Cirad : Axe 3 (2014-2018) - Alimentation durable

Auteurs et affiliations

• Campbell Katrina, Queen Mary University of London (GBR)
• Ferreira Cavalcante Ana L., Queen Mary University of London (GBR)
• Galvin-King Pamela, Queen Mary University of London (GBR)
• Oplatowska-Stachowiak Michalina, Queen Mary University of London (GBR)
• Brabet Catherine, CIRAD-PERSYST-UMR Qualisud (FRA)
• Metayer Isabelle, CIRAD-PERSYST-UMR Qualisud (FRA)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)
• Haughey Simon A., Queen Mary University of London (GBR)
• Elliott Christopher T., Queen Mary University of London (GBR)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/583534/)

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