Effect of post-harvest management practices on the mycobiome and ochratoxin A contamination of differently processed Robusta coffees from Ivory Coast

Lopez Rodríguez Claudia, Strub Caroline, Chochois Vincent, Verheecke-Vaessen C., Durand Noël, Jourdan Christophe, Fontana Angélique, Guehi Tagro Simplice, Medina Angel, Schorr-Galindo Sabine. 2023. Effect of post-harvest management practices on the mycobiome and ochratoxin A contamination of differently processed Robusta coffees from Ivory Coast. Postharvest Biology and Technology, 206:112573, 9 p.

https://doi.org/10.1016/j.postharvbio.2023.112573

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Résumé : Ochratoxin A (OTA) is a secondary metabolite produced primarily by the genus Aspergillus sp. sections Circumdati and Nigri. It can accumulate in coffee at post-harvest stage. In the present study five different dry processed coffees were sampled from Ivory Coast at harvesting and after drying. The OTA was detected in all the samples, from 3.62 μg/kg of coffee just after harvest to a higher-level of contamination in the dried coffee, between 11.04 and 760.24 μg/kg of coffee. Metabarcoding was used to further study the changes in the mycobiota. The alpha and beta diversity analysis revealed the presence of unique and more diverse fungal communities on the coffee after drying compared to fresh harvested coffee. Specially, coffee dried for a longer period showed a higher diversity. Fungal species such as Hypopichia sp. or Bernettozyma sp., were replaced in the top ten abundant species after drying by species such as Kurtzmaniella sp., Meyerozyma sp. and Fusarium sp. The increasing abundance of the ochratoxigenic fungi, A. carbonarius was correlated to the increased concentration of OTA on coffee. The construction of the negative correlation network shows that some yeasts could be good candidates for the biocontrol of A. carbonarius. The combination of DNA metabarcoding and OTA quantification was effective at deciphering the post-harvest origin of the OTA contamination. Overall, this study highlights the changes in the mycobiota under different drying conditions. Future perspectives include developing actions of prevention and control of the contamination with OTA during the post-harvest stages.

Mots-clés Agrovoc : ochratoxine, fève de café, contamination des aliments

Mots-clés géographiques Agrovoc : Côte d'Ivoire

Mots-clés libres : Metagenomics, Ochratoxin A, Robusta coffee, Toxigenic fungi, Post-harvest management, Coffee safety

Classification Agris : Q03 - Contamination et toxicologie alimentaires
Q02 - Traitement et conservation des produits alimentaires
J10 - Manutention, transport, stockage et conservation des produits agricoles

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Agences de financement hors UE : Agropolis Fondation, Cranfield University

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