Improving the triphenylphosphine/triphenylphosphine oxide (TPP/TPPO)-basedmethod for the absolute and accurate quantification by FTIR-ATR of hydroperoxides in oils or lipid extracts

Wind Juliette, Villeneuve Pierre, Taty Mignon Prince Exaucé, Figueroa-Espinoza Maria-Cruz, Barea Bruno, Pradelles Rémi, Durand Erwann. 2024. Improving the triphenylphosphine/triphenylphosphine oxide (TPP/TPPO)-basedmethod for the absolute and accurate quantification by FTIR-ATR of hydroperoxides in oils or lipid extracts. European Journal of Lipid Science and Technology, 12 p.

Résumé : Research on natural sources of polyunsaturated fatty acids (PUFAs) for both food and nutraceutical prospects has significantly grown in recent years. Some plant oils and lipid extracts contain carotenoids, xanthophylls, sterols, and/or phenolic compounds that can provoke a lipid hydroperoxides (LOOHs) overestimation when quantifying them using nonselective colorimetric assays. Herein, we have optimized a mid-infrared method using Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR) to quantify LOOHs in oils or lipid extracts. This method is based on the conversion of triphenylphosphine (TPP) to triphenylphosphine oxide (TPPO) in the presence of hydroperoxides, with a direct assessment of TPPO levels following the formation of an oil film on the ATR crystal's surface, allowing for a low detection limit of 0.5 mmol of LOOH kg−1. The concentration of oil and TPP, as well as the reaction time, were optimized. It was demonstrated that the presence of pigments, unsaponifiable compounds, phenolics, and/or PUFAs on oils, do not disrupt the analysis. Furthermore, the stoichiometry of the TPP/LOOH reaction was examined, confirming the reliability of the method in detecting various forms of hydroperoxides. An accelerated oxidation study was carried out and the hydroperoxide contents measured using TPP/TPPO were found to be comparable to those obtained using the ferric thiocyanate method. Our method offers a fast, simple, robust, and sensitive approach to accurately quantify hydroperoxides, regardless of the chemical composition of oil or lipid extracts. Practical Application: The hydroperoxide assay method outlined in this study allows for the rapid and straightforward detection of hydroperoxides in pure oil matrices. The method's elevated sensitivity facilitates the early identification of oxidation indicators in oils, especially those with significant carotenoid or xanthophyll contents since these compounds may affect the results when using methods based on colorimetric quantification of hydroperoxides. This accurate, precise, and reproducible approach requires only small quantities of test samples and chemical products, making it well suited to routine application in laboratories and industrial environments.

Mots-clés Agrovoc : composition chimique, composé phénolique, caroténoïde, stérol, acide gras, extrait d'origine végétale, oxydation, huile de colza, huile d'olive, huile végétale, lipide

Mots-clés complémentaires : Huile de noix

Mots-clés libres : Lipid oxidation, Infra Red, Oils

Classification Agris : Q01 - Sciences et technologies alimentaires - Considérations générales
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Agences de financement hors UE : Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Auteurs et affiliations

• Wind Juliette, CIRAD-PERSYST-UMR Qualisud (FRA)
• Villeneuve Pierre, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-1685-1494
• Taty Mignon Prince Exaucé, Université de Montpellier (FRA)
• Figueroa-Espinoza Maria-Cruz, Université de Montpellier (FRA)
• Barea Bruno, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0009-0007-5164-1632
• Pradelles Rémi, Microphyt (FRA)
• Durand Erwann, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-0306-8081 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/609912/)

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