Transposable element accumulation drives genome size increase in Hylesia metabus (Lepidoptera: Saturniidae), an urticating moth species from South America

Perrier Charles, Allio Rémi, Legeai Fabrice, Gautier Mathieu, Bénéluz Frédéric, Marande William, Theron Anthony, Rodde Nathalie, Herrera Melfran, Sauné Laure, Parrinello Hugues, McClure Melanie, Arias Monica. 2024. Transposable element accumulation drives genome size increase in Hylesia metabus (Lepidoptera: Saturniidae), an urticating moth species from South America. Journal of Heredity:esae069, 10 p.

https://doi.org/10.1093/jhered/esae069

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Url - jeu de données - Entrepôt autre : https://bipaa.genouest.org/sp/hylesia_metabus/

Résumé : We present the first nuclear genome assembly and a complete mitogenome for Hylesia metabus (Arthropoda; Insecta; Lepidoptera; Saturniidae). The assembled nuclear genome sequence is 1,271 Mb long, which is among the 10 largest lepidopteran genome assemblies published to date. It is scaffolded in 31 pseudo chromosomes, has a BUSCO score of 99.5%, and has a highly conserved synteny compared with phylogenetically close species. Repetitive elements make up 67% of the nuclear genome and are mainly located in intergenic regions, among which LINEs were predominant, with CR1-Zenon being the most abundant. Phylogenetic and comparative analyses of H. metabus assembly and 17 additional Saturniidae and Sphingidae assemblies suggested that an accumulation of repetitive elements likely led to the increased size of H. metabus' genome. Gene annotation using Helixer identified 26,122 transcripts. The Z scaffold was identified using both a synteny analysis and variations of coverage for two resequenced male and female H. metabus. The H. metabus nuclear genome and mitogenome assemblies can be found and browsed on the BIPAA website and constitute useful resources for future population and comparative genomics studies.

Mots-clés Agrovoc : génome, Saturniidae, Hylesia, Insecta, adn, génomique, Sphingidae, Transcription génique, séquence nucléotidique

Mots-clés géographiques Agrovoc : France, Guyane française

Mots-clés libres : Ashen moth, Lepidopterism, Population outbreaks, Repetitive elements, Yellowtail moth

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