Rapid quantitative determination of maltose and total sugars in sweet potato (Ipomoea batatas L. [Lam.]) varieties using HPTLC

Lebot Vincent. 2017. Rapid quantitative determination of maltose and total sugars in sweet potato (Ipomoea batatas L. [Lam.]) varieties using HPTLC. Journal of Food Science and Technology, 54 (3) : 718-726.

https://doi.org/10.1007/s13197-017-2510-2

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Quartile : Q2, Sujet : FOOD SCIENCE & TECHNOLOGY

Résumé : When a raw sweet potato root is analysed, only sucrose, glucose and fructose are present but during cooking, starch is hydrolysed into maltose giving the sweet flavour to cooked roots. This study aimed at developing an HPTLC protocol for the rapid quantitative determination of maltose and total sugars in four commercial varieties and to compare them to 243 hybrids grouped by flesh colour (white, orange, purple). In commercial varieties, mean maltose content varied from 10.26 to 15.60% and total sugars from 17.83 to 27.77% on fresh weight basis. Hybrids showed significant variation in maltose content within each group, with means ranging from 7.65% for white-fleshed, to 8.53% in orange- and 11.98% in purple-fleshed. Total mean sugars content was 20.24, 22.11 and 26.84% respectively for white, orange and purple flesh hybrids. No significant correlations were detected between individual sugars but maltose and total sugars content were highly correlated. Compared to the best commercial variety (Baby), 25 hybrids (10.3%) presented a higher maltose content and 40 (16.5%) showed a higher total sugars content. HPTLC was observed as an attractive, cost efficient, high-throughput technique for quantitating maltose and total sugars in sweet potatoes. Perspectives for improving sweet potato quality for consumers' requirements are also discussed.

Mots-clés Agrovoc : patate douce, Ipomoea batatas, maltose, teneur en glucides, analyse quantitative, variété, technique analytique, composition chimique, composition des aliments, chromatographie en couche mince

Mots-clés géographiques Agrovoc : Vanuatu

Mots-clés libres : Maltose, HPTLC, Patate douce, Ipomoea batatas

Classification Agris : Q04 - Composition des produits alimentaires
F60 - Physiologie et biochimie végétale
U30 - Méthodes de recherche
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 3 (2014-2018) - Alimentation durable

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