Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean

Chen Nicolas W.G., Serres-Giardi Laurana, Ruh Mylène, Briand Martial, Bonneau Sophie, Darrasse Armelle, Barbe Valérie, Gagnevin Lionel, Koebnik Ralf, Jacques Marie Agnès. 2018. Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean. BMC Genomics, 19:606, 20 p.

https://doi.org/10.1186/s12864-018-4975-4

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Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : GENETICS & HEREDITY

Résumé : Background: Host specialization is a hallmark of numerous plant pathogens including bacteria, fungi, oomycetes and viruses. Yet, the molecular and evolutionary bases of host specificity are poorly understood. In some cases, pathological convergence is observed for individuals belonging to distant phylogenetic clades. This is the case for Xanthomonas strains responsible for common bacterial blight of bean, spread across four genetic lineages. All the strains from these four lineages converged for pathogenicity on common bean, implying possible gene convergences and/or sharing of a common arsenal of genes conferring the ability to infect common bean. Results: To search for genes involved in common bean specificity, we used a combination of whole-genome analyses without a priori, including a genome scan based on k-mer search. Analysis of 72 genomes from a collection of Xanthomonas pathovars unveiled 115 genes bearing DNA sequences specific to strains responsible for common bacterial blight, including 20 genes located on a plasmid. Of these 115 genes, 88 were involved in successive events of horizontal gene transfers among the four genetic lineages, and 44 contained nonsynonymous polymorphisms unique to the causal agents of common bacterial blight. Conclusions: Our study revealed that host specificity of common bacterial blight agents is associated with a combination of horizontal transfers of genes, and highlights the role of plasmids in these horizontal transfers.

Mots-clés Agrovoc : phylogénie, Xanthomonas, transfert de gène, pathologie végétale, Xanthomonas campestris citri, séquence nucléotidique, génome, virus des végétaux, haricot commun, lignée, champignon pathogène, génie génétique, gène

Mots-clés géographiques Agrovoc : France, La Réunion

Mots-clés libres : Xanthomonas, Common bean, TAL effectors, Host adaptation, Horizontal gene transfer

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Etude comparative des génomes et des transcriptomes de Xanthomonas phytopathogènes, (FRA) Organisation et montée en puissance d'une Infrastructure Nationale de Génomique

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